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flimfit / FLIMfit

State of the art fluorescence lifetime imaging analysis software
http://flimfit.org
GNU General Public License v2.0
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FLIMfit will not load a .omeTIFF file from a Leica DMi8 #386

Open kdbuchan1 opened 2 years ago

kdbuchan1 commented 2 years ago

Hi there,

I'm having trouble importing a .ometiff file from a Leica DMi8 microscope. I also tried opening in a picoquant file but I can't seem to fit the IRF decay properly. The IRF never seems to be implemented properly.

If you can get back to me that would be amazing. I'm trying to finish off a publication and I only have a few weeks to get it together.

Thank you! Kyle

Error using flim_data_series/get_image_dimensions (line 185) Java exception occurred: java.lang.IndexOutOfBoundsException: Index: 1, Size: 1 at java.util.ArrayList.rangeCheck(Unknown Source) at java.util.ArrayList.get(Unknown Source) at ome.xml.model.Pixels.getChannel(Pixels.java:628) at ome.xml.meta.OMEXMLMetadataImpl.getChannelName(OMEXMLMetadataImpl.java:1211)

Error in flim_data_series/load_multiple (line 30)

Error in flim_data_series/load_single (line 104)

Error in flim_data_series_controller/load_single (line 168)

Error in front_end_menu_controller/menu_file_load_single_callback (line 656)

Error in front_end_menu_controller>@(varargin)obj.menu_file_load_single_callback(varargin{:})

Error in EC (line 7)

Error in front_end_menu_controller>@(x,y)EC(fcn) (line 263)

seanwarren commented 2 years ago

Hi Kyle,

From a Leica SP8 you need to export your data as a PT3 file for analysis in FLIMfit. An OME TIFF from the Leica software does not contain the time resolved data.