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flimfit / Galene

A tool for compensating for motion in FLIM data
GNU General Public License v2.0
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Using Galene on augmented data #21

Closed akatav closed 4 years ago

akatav commented 4 years ago

So, i have 2 photon fluorescence image of many dendrites and synapses (in 2 dimension). Due to raster scanning, each image has motion artifacts from breathing and head movement and heartbeats. My problem is right now, there is no time lapse of the FOV, ie, the FOV is raster scanned only once. So, if i augment this image (by affine translations, cropping, rotation etc) and feed it to Galene, can i expect motion corrected image as output ?

seanwarren commented 4 years ago

Hello, Galene works by using temporal information to correct for motion so you need a timelapse image. One option is to increase your scan rate and acquire time lapses with lower signal which you can then correct in Galene to a single, corrected image