Open pjbriggs opened 1 year ago
Proposal to strip any trailing Ns from index sequences in sample sheets in the make_fastqs command/pipeline. For example:
N
make_fastqs
SM1,SM1,,,,,CCGCGGTTNNNNNNN,,CTAGCGCT,Test,
should be transformed to
SM1,SM1,,,,,CCGCGGTT,,CTAGCGCT,Test,
While BaseSpace treats Ns as matching any base, bcl2fastq cannot handle the presence of Ns.
bcl2fastq
Proposal to strip any trailing
Ns from index sequences in sample sheets in themake_fastqscommand/pipeline. For example:should be transformed to
While BaseSpace treats
Ns as matching any base,bcl2fastqcannot handle the presence ofNs.