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fmi-faim / napari-psf-analysis

A napari plugin to analysis point spread functions.
https://fmi-faim.github.io/napari-psf-analysis/
BSD 3-Clause "New" or "Revised" License
8 stars 3 forks source link

Gaussian fitting in 1D seems to give too large values #15

Closed lgelman closed 2 years ago

lgelman commented 2 years ago

20220829_Bead_1_FullseqTIRF-GFP-Cy5mCherryWithSMB.zip 20220607_Bead_1_FullseqTIRF-GFP-Cy5mCherryWithSMB.zip

lgelman commented 2 years ago

I uploaded teh original files as well as the result of the analysis with the ImageJ Macro. I wish the values for the Gaussian fit in x and y in teh napari plugin were very close to those obtained with ImageJ. For this, the fit for x and y should be done in 2D in one plan, at the center of the bead, as this would reflect the reoslution of the scope when one one plane is acquired.

lgelman commented 2 years ago

20220829_Bead_1_FullseqTIRF-GFP-Cy5mCherryWithSMB.stk_29-8-2022_TIRF_100x_1.49 FWHMa=1841nm FWHMl=339nm.tif.zip 20220607_Bead_1_FullseqTIRF-GFP-Cy5mCherryWithSMB.stk_07-6-2022_TIRF_100x_1.49 FWHMa=611nm FWHMl=291nm.tif.zip

tibuch commented 2 years ago

Should be resolved with the new version.