hello .i have error while align using reademption. im sure about the requirements that i have fullfilled .please check the screenshoots and point me the mistake and solution.

foerstner-lab / READemption

A pipeline for the computational evaluation of RNA-Seq data

https://reademption.readthedocs.io

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hello .i have error while align using reademption. im sure about the requirements that i have fullfilled .please check the screenshoots and point me the mistake and solution. #47

Open AzkaSaleem opened 1 year ago

AzkaSaleem commented 1 year ago

Screenshot (7) Screenshot (8) Screenshot (6)

AzkaSaleem commented 1 year ago

even IGB aligned my .fa and .gff that means i have same header in fasta asgff3 first colum .the input directory is also all set . why it is asking me for bam file

Tillsa commented 1 year ago

Hi @AzkaSaleem Please add the option --fastq when running the align subcommand. This option needs be turned on when aligning fastq files. Kind regards, Till