foerstner-lab / READemption

A pipeline for the computational evaluation of RNA-Seq data
https://reademption.readthedocs.io
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Assign libraries for paired end data in the reademption deseq command #61

Open termithorbor opened 2 months ago

termithorbor commented 2 months ago

Hi,

how do I assign the libraries for paired end data in the reademption deseq command?

The names of my reads are like this:

1-hour-1A_p1.fq 1-hour-1A_p2.fq 1-hour-2A_p1.fq 1-hour-2A_p2.fq

Where p1 are the forward and p2 the reverse reads.

Do I have to wirte: a) --libs 1-hour-1A,1-hour-2A or b) --libs 1-hour-1A_p1.fq,1-hour-1A_p2.fq,1-hour-2A_p1.fq,1-hour-2A_p2.fq

I would assume a) but a statement in the manual would be very helpful since the examples are solely for single end data.

Thanks in advance, Thorben

Tillsa commented 2 months ago

Hello @termithorbor

It is option a)

And thank you for pointing out that the documentation is missing a paired end data example, we will fix this.

Best, Till