friedlanderlab / mirtrace

miRTrace
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PHRED score handling examples missing / unclear behaviour #12

Open apeltzer opened 7 months ago

apeltzer commented 7 months ago

This code snippet here behaves very odd. You cannot for example specify a global adapter, but then having PHRED scores specified. There is also no global option for PHRED scores (although most people sequence a single FC, then having PHRED scores the same for all samples on it...) - would it be possible to:

We had data now that had overall PHRED=38 scores, so the tool failed because it considered this to be "too good to be true" (although its verifiable reality...).

x-ref https://github.com/nf-core/smrnaseq/pull/342/files