Running nanomonsv with WGS samples

[waltergallegog]

Hello, I'm running nanomonsv on a tumor and control .bam files from a WGS run. The .bam files are 63GB and 85GB respectively.

• Have you run nanomonsv with samples of this size (or larger than 50GB)?
• If yes, is it possible for you to share some details about the specs of the used computer, the run time required for the parse and get commands, and the RAM used? I just want to have an estimation of how much it could take in my case.
• Is it safe if I execute two instances of nanomonsv parse, so I can parse both tumor and ctrl at the same time? (I mean, if the program is perhaps using temp files that could get mixed/corrupted if two instances run at the same time)

Thanks Walter.

[friend1ws]

Sorry for the delayed response.

Have you run nanomonsv with samples of this size (or larger than 50GB)? Yes.

If yes, is it possible for you to share some details about the specs of the used computer, the run time required for the parse and get commands, and the RAM used? I just want to have an estimation of how much it could take in my case.

I believe RAM used is less than 8GB. So e.g. m5.large of AWS EC2 or m5.xlarge would be enough.

Is it safe if I execute two instances of nanomonsv parse, so I can parse both tumor and ctrl at the same time? (I mean, if the program is perhaps using temp files that could get mixed/corrupted if two instances run at the same time)

That's would be enough if you can separate the computational environment.

[waltergallegog]

Thanks for the information, I was able to run nanomonsv with my WGS samples without any trouble.