Hi, I seem to have the same issue as described here in that simreads just seems to run without ever completing - my current run has been going for 5 days. This is a ~400Mb genome, and I'm using SURVIVOR version 1.0.7.
I used this command to generate an error profile from the alignment of ONT reads against the genome
Hi, I seem to have the same issue as described here in that simreads just seems to run without ever completing - my current run has been going for 5 days. This is a ~400Mb genome, and I'm using SURVIVOR version 1.0.7.
I used this command to generate an error profile from the alignment of ONT reads against the genome
samtools view -s 0.1 HMW_127_minimap.sorted.bam | SURVIVOR scanreads 1000 HMW_127_error_profile.txt
Then used the error profile in conjunction with the genome like so:
SURVIVOR simreads genome.fasta HMW_127_error_profile.txt 20 006_sim
The head of my error profile looks like this:
And the tail like this:
Any advice would be much appreciated - thank you.