I am facing a platform that output Barcode in reverse-complemented form with Illumina sequencing primers. (the barcode will be normal with their own primer which is on the other side)
It will be helpful if fgbio can reverse-complement a read structure, for example, when the type is in lower case.
Will you consider make '30b+S10M' to work as the first 30 bp need to be reverse-complemented and then be used as barcode, and the last 10 bp to directly used as UMI ?
I am facing a platform that output Barcode in reverse-complemented form with Illumina sequencing primers. (the barcode will be normal with their own primer which is on the other side) It will be helpful if
fgbiocan reverse-complement a read structure, for example, when the type is in lower case.Will you consider make '30b+S10M' to work as the first 30 bp need to be reverse-complemented and then be used as barcode, and the last 10 bp to directly used as UMI ?