hello,
I runned fgbio's FilterConsensusReads to my RNAseq data, but the output results files are of too little size.
The raw read uBAM file had 3-4GB,
after first mapping and quality control, there had 2GB data,
this is one of the tag family size histogram file.
my issue is this, after I CallConsensusReads and FilterConsensusReads, is seems most of reads were filtered out, just 38kB data
I tried increase the umi from 5 to 6, and it didn't work, if someone know how to sovle this problem?
hello, I runned fgbio's FilterConsensusReads to my RNAseq data, but the output results files are of too little size. The raw read uBAM file had 3-4GB, after first mapping and quality control, there had 2GB data, this is one of the tag family size histogram file.![image](https://github.com/fulcrumgenomics/fgbio/assets/126665257/1ed1bbee-5f90-460d-a68b-a016254f4ae2)
my issue is this, after I CallConsensusReads and FilterConsensusReads, is seems most of reads were filtered out, just 38kB data
I tried increase the umi from 5 to 6, and it didn't work, if someone know how to sovle this problem?