I am currently trying to use FRASER2 to analyse RNAseq data (mRNA and totalRNA), from muscle biopsy samples. I have been trying to reproduce the vignette on my data and I was surprised to see that all my adjusted p-values were all at 1. It seems that the vignette is also showing adjusted p-values at 1. I was wondering why this is, and how can this be resolved? The data has been tested already and we know that there are outliers in certain samples.
I am running the script on R 4.4.0 and the installed FRASER version is 2.0.0.
Dear FRASER2 team,
I am currently trying to use FRASER2 to analyse RNAseq data (mRNA and totalRNA), from muscle biopsy samples. I have been trying to reproduce the vignette on my data and I was surprised to see that all my adjusted p-values were all at 1. It seems that the vignette is also showing adjusted p-values at 1. I was wondering why this is, and how can this be resolved? The data has been tested already and we know that there are outliers in certain samples.
I am running the script on R 4.4.0 and the installed FRASER version is 2.0.0.
Thank you very much in advance! Camille