galaxy001 / pirs

profile basd Illumina pair-end Reads Simulator
https://code.google.com/p/pirs/
GNU General Public License v2.0
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Mean quality values by position identical between first and second read #10

Open schmeing opened 4 years ago

schmeing commented 4 years ago

I observed that in the current version, the mean of the quality values at each read position is identical between first and second read. This is not the case for the reads I used to train the quality distributions. This happens for all tested datasets.

I trained the distribution with: baseCalling_Matrix_calculator -m 42 -r ref.fa -i reads.bam -o baseCalling

And included it during the simulation with -B baseCalling.count.matrix

galaxy001 commented 4 years ago

We do not distinguish read 1 and read 2 yet.

In some further future, I may rewrite the whole thing.