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galaxyproject / dunovo

Reference-free duplex sequencing pipeline.
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Barcode correction: Set allowed edit distance during bowtie2 alignment as well as in correct.py #5

Closed NickSto closed 6 years ago

NickSto commented 7 years ago

With high allowed edit distances, bowtie2 can be the bottleneck, filtering out alignments with lower edit distances than you're allowing in correct.py.

NickSto commented 6 years ago

Fixed in 22fa52c (by switching to bowtie).