Major changes to allow input of multiplexed single-cell data, with all barcoded and UMI-tagged reads in a single file, rather than separating into separate input files for each cell. PCR duplicates are removed by discarding any reads for which the same barcode and UMI are mapped to the same transcript.
Major changes to allow input of multiplexed single-cell data, with all barcoded and UMI-tagged reads in a single file, rather than separating into separate input files for each cell. PCR duplicates are removed by discarding any reads for which the same barcode and UMI are mapped to the same transcript.