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Size is incorrect #1

Closed bwpearre closed 7 years ago

bwpearre commented 7 years ago

Scanimage reports the FOV of the lens is 270 μm on each side. Printing full-FOV cubes results in 500-μm-on-a-side cubes. They do appear to be about 270 μm tall... maybe? Anyway, fix the reported FOV!

bwpearre commented 7 years ago

I believe that 7e6e07e75241e363b4a5b498bbcc12040e6c2e22 will help with this, and also the "objectiveResolution" parameter that is controlled in the machine config file.

bwpearre commented 7 years ago

Ongoing problem, largely resolved with Christos's 2-micron ruler (printed on the NanoScribe, and verified via SEM). Procedure: image the item, which has known size. This results in an image like the attached. It has spikes every 10 microns, and big spikes every 100 microns; the stair steps on each spike are at 2-micron intervals. Use this to set the objectiveResolution parameter in Machine_Data_File: ask for the FOV from hSI.hRoiManager.imagingFovUm, and multiply objectiveResolution until it matches what you see on the ruler...? ruler

bwpearre commented 7 years ago

This will be somewhat of an ongoing problem, but Christos's ruler basically fixes this. See above.