Closed FrederikTheisen closed 3 months ago
Hi,
From what I'm understanding, you have two independent exchange processes going on, but they share a common state. I believe this would be best described by an off-pathway 3-state where B <-> A <-> C, and you would set B <-> C rates to 0.
In the scenario where you have two chemically identical states A that slowly exchange, I don't think there's any way to experimentally see that. Perhaps if you run an EXSY experiment, you may see a cross-peak between B and C states, if they're populated enough to even see them, but presumably they are not which is why you are using CEST.
As for your 4-state alternative, I would need to run some simulations, but I think that just boils down to the 3-state mentioned above. I'm not sure the data can accurately capture differences between the two options.
Those are my 2 cents anyways.
Hi thanks for your response.
To clarify, this is proline cis/trans isomerization, with a 30% cis population and thus very visible and assignable in NMR. The model is only speculation, and I'm currently producing a mutant (removing the proline) which will result in only one ground state.
The shared peak position is thus not a common state, but contains what is effectively two different molecules. There is an equilibrium constant between the two molecules, but the Kex is << 1. Thus I have two indenpendent exchange processes that share an exact peak position. I know the equilibrium constant between the two ground states and thus also the intensity ratio between the two.
If I modeled this using 3 states, state A would contain a mix of two different molecules.
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Hi
I'm trying to analyze some CEST data where I have two different unbound states in very slow exchange (basically zero exchange) with the same chemical shift in a 15N-HSQC. Upon binding, the two states will differ and thus a single peak shows three-state behavior, even though it is actually 2 x two-state behavior. I know the Keq of the two unbound states.
While the data fits a three-state model, I'm not sure the output values are meaningful since the ground state effectively contains two different molecules.
What I need is a four-state model, with two binding events (A->B and C->D) and potentially two very slow isomerization event (A->C and B->D). But since my observed peak contains both A and C state, I need to detect saturation of both the A state and the C state.
I've implemented this by duplicating the 15N-CEST experiment and changing the detect function to return "[iz_a] + [iz_c]" which appears to work.
My question is if this is a reasonable thing to do?
Thanks. Frederik