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gear-genomics / tracy

Basecalling, alignment, assembly and deconvolution of Sanger Chromatogram trace files
https://www.gear-genomics.com/
BSD 3-Clause "New" or "Revised" License
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basecall problem #91

Open yuanzhao0502 opened 1 month ago

yuanzhao0502 commented 1 month ago

I have a ab1 file tracy basecall only generate 121 bases but it visualized normal after that. What's the reason tracy can not doing the basecalling after that? Thank you for help!

7E983462-3D65-4fdf-AB43-0169030AC508

zip format of ab1 file

tobiasrausch commented 4 weeks ago

Tracy requires the original basecall positions from the Applied Biosystem machine. I guess in your case the machine terminated the Autoanalysis prematurely and thus, I would suggest to re-run the sequencing analysis on the machine.

yuanzhao0502 commented 3 weeks ago

Tracy requires the original basecall positions from the Applied Biosystem machine. I guess in your case the machine terminated the Autoanalysis prematurely and thus, I would suggest to re-run the sequencing analysis on the machine.

Thanks a lot for your suggest!

Actually I have a try to use biopython to read this file. "record.letter_annotations" can identify 972 bases. What's the differences of basecalling method between tracy and biopython?