COSMOS fitting seems to depend on the number of channels used for processing

gelles-brandeis / tapqir

Bayesian analysis of co-localization single-molecule microscopy image data.

Apache License 2.0

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COSMOS fitting seems to depend on the number of channels used for processing #423

Open jc-brandeis opened 11 months ago

jc-brandeis commented 11 months ago

COSMOS fitting on a set of 2-channel data gives different results when the 2 channels are processed separately or together: spot detection in channel 1 shows very low p_specific for all AOIs in all frames when processed together with channel 2. spot detection in channel 1 shows reasonable p_specific when processed by itself.

ordabayevy commented 11 months ago

A shared parameter between two channels when analyzed together is proximity. I wonder if the second channel influences the value of proximity parameter which in turn affects the p_specific for the first channel.