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gencorefacility / reform

Modify existing reference fasta and gff3/gtf files to include a new sequence
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New Chromosome #7

Closed JBreunig closed 4 years ago

JBreunig commented 4 years ago

Wonderful work on this! It worked well for me in terms of inserting into a chromosomal locus.

However, is there a way to insert a "new" chromosome (i.e. just for transgenic elements--EGFP, TdTomato, etc.)?

tilofrei commented 2 years ago

To have it in a "new" chromosome you could just manually append the GFP component to the genome.fa file as outlined here: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_mr