IPR002717

[Antonialock]

Hello,

pombe mst1 has a mapping to regulation of transcription, DNA-templated from IPR002717.

I can't see any evidence that mst1 is regulating transcription directly. Mst1 is a histone acetyltranferase.

"In this study, our direct measurements of chromatin compaction in the meiotic horsetail nucleus of living S. pombe cells demonstrated that chromatin decompaction occurs during meiotic DNA replication. Our results also show that this decompaction is due to DNA replication-coupled acetylation of histone H4 by Mst1." -PMID:26223950

"H3K4ac, mediated by Mst1, is enriched at pericentromeres concomitantly with heterochromatin reassembly....H3K4ac mediates a switch to allow heterochromatin reassembly." PMID:20299449

In this paper PMID:20299455 they show that expression of a gene is controlled by H4 modifications, mediated by mst1 and png1. The authors seem to think that png1 regulates transcription and acetylation, and mst1's jobs is to alter chromatin compaction. @ValWood ?

[ValWood]

I'm not sure early in vitro assays of NuA4 subunits appear to show stimulation of transcription in viro, but I guess that could be a non physiological observation, as so many of the complex members do have a role in transcription as members of other complexes...

I always thought NuA4 was DSB repair and that is how it is described in GO right now. AFAIK, mst is only a member of NuA4 so far....

So based on this I would agree...

https://www.yeastgenome.org/reference/S000046619

[pgaudet]

@asangrador Can you please have a look ?

Thanks, Pascale

[asangrador]

This term was added after several proteins in UniProt and publications, for example O95251, Histone acetyltransferase KAT7/HBO1 (PMID:18832067; HBO1 histone acetylase is a coactivator of the replication licensing factor Cdt1 ), and Q08649 (PMID:11036083, The yeast NuA4 and Drosophila MSL complexes contain homologous subunits important for transcription regulation). In the most recent paper I can find about NuA4 (PMID:29145630) it mentions "the acetyltransferase complex NuA4 that acetylates histones H4, H2A and H2A.Z and regulates transcriptional and DNA repair programs". So it looks like is involved in transcriptional regulation?

Amaia

[pgaudet]

@sylvainpoux you have looked at these proteins a lot - do you have anything to add to help on this ?

Thanks, Pascale

[ValWood]

I thin NuA4 probably does have a real role in transcription. it must be more than repair because 8 subunits are essential for vegetative growth....

And the wikipedia article refers to to transcriptional acitivites...