ValWood
commented
4 years ago These are 3 splicing complex components conserved across eukaryotes. The evidence seems to be largely genetic suppression experiments and the conclusions also don't appear to implement in this pathway signalling.
MOS4 PMID:17575050 CDC5 PMID:17575050 PRL1 PMID:17575050 MOS4 (Modifier Of snc1, 4), AtCDC5, and PRL1 (Pleiotropic Regulatory Locus 1)--that are all essential for plant innate immunity. Since the complex components along with their interactions are highly conserved from fission yeast to Arabidopsis and human, they may also have a yet-to-be-identified function in mammalian innate immunity. In fission yeast, cerevisiae human and arabidopsis these are all spicing factors, so this seems to be indirect?
This is not signalling, from the conclusion It is reasonable to propose that the Arabidopsis MAC is orthologous to the NTC, which has been indicated to be essential for splicing (Ajuh et al. 2001), based on studies in human and yeast. Since the majority of plant genes are interrupted by noncoding introns, a defect in general splicing of mRNA would likely result in catastrophic phenotypes or death. For example, several general splicing mutants are embryo lethal, based on data in the SeedGenes database (Tzafrir et al. 2003). Since mutations in MOS4, PRL1, or AtCDC5 alone only have minor effects on the plants’ normal growth and development, it is unlikely that the protein products of these genes are essential components of splicing. However, since mos4-1 Atcdc5-1 and mos4-1 prl1-1 double-homozygous mutants seem to be lethal, MAC as a whole could be required for an essential process, such as spliceosome assembly, as suggested from studies in yeast and humans (Tarn et al. 1993; Grillari et al. 2005) and ……Although the functional relevance of alternative splicing in innate immunity is unknown, the association of the NTC with the spliceosome led us to hypothesize that MOS4, AtCDC5, and PRL1 may be involved in alternative splicing. We tested several alternatively spliced genes, including RPS4, AtSRp30, AtSRp34, U1snRNP, ANP1, and POT1, for relative levels of transcript variants of each in the mos4-1, Atcdc5-1, and prl1-1 mutants by real-time RT–PCR. In all cases, there were no significant differences in transcript variant levels between wild type and the mos4-1, Atcdc5, and prl1 mutants (Fig. 7). Our data suggest that MOS4, AtCDC5, and PRL1, while possibly associated with a spliceosome complex, are individually not involved in general or alternative RNA splicing. We cannot discount the possibility that the intact MAC complex is required for spliceosome assembly as the NTC is in yeast, or that the individual members regulate the splicing of an unknown RNA species.
https://www.uniprot.org/uniprot/Q42384 cwf15 cdc5
For this one, if you approve the changes, this date seems to be transferred into keywords. There are a few other spurious mappings on these proteins. I can look at these afterwards. (not to self, also an Interpro DNA binding, possibly from Myb)
pgaudet
tberardini
EricaBakker
EDITED: Annotation review spreadsheet is here: https://docs.google.com/spreadsheets/d/1fv8c9WDOxFJ0CILQNjpyCfgjy1yAj09eMCEYgqP4Zy0/edit#gid=0
from
https://github.com/geneontology/go-ontology/issues/18587
FMO1 is FMO1 PMID:16856982 we showed that the enhanced basal resistance phenotype was dependent on the accumulation of salicylic acid. FMO1-3D plants showed wild-type resistant reactions after inoculation with avirulent bacteria, indicating that the R-gene-mediated defence physiology was not compromised by FMO1 over-expression. We discuss the possibility that the FMO may participate in the detoxification of virulence factors produced by pathogens.
Moreover, while CDR1 and ADR1 are believed to be involved in pathogen defence signalling, FMO1 encodes an FMO. Flavin‐containing mono‐oxygenases probably occur in all life forms, as they have been detected in bacteria, fungi, plants and animals (Schlenk, 1998). Mostly studied in animal systems, they have been shown to be important for the detoxification of xenobiotica, i.e. molecules that are foreign to the organism
Q9LMA1
FMO1 GO:0009870 defense response signaling pathway, resistance gene-dependent PMID:16531493
and GO:0010204 defense response signaling pathway, resistance gene-independent PMID:16856982
This one is strange because it is FMO1 encodes an FMO. Flavin‐containing mono‐oxygenases probably occur in all life forms, as they have been detected in bacteria, fungi, plants and animals (Schlenk, 1998). Mostly studied in animal systems, they have been shown to be important for the detoxification of xenobiotica, i.e. molecules that are foreign to the organism
It does not appear to be signalling at all?