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This repository hosts the tracker for issues pertaining to GO annotations.
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PTN000117598 not a ribosomal subunit - PTHR11075 #3646

Closed ValWood closed 3 years ago

ValWood commented 3 years ago

pth4 is a translation release factor, not a ribosomal subunit

GO:0005762 | mitochondrial large ribosomal subunit | IBA with S000005474 , Q14197 , pth4 , PTN000117598

pgaudet commented 3 years ago

In human this subunit has been found to be in the ribosome by xray crystallography

https://europepmc.org/article/MED/25278503 Is this really inconsistent that a ribosomal subunit would act as a release factor?

ValWood commented 3 years ago

It is a tRNA release factor associated with the mitochondrial ribosome. It is ribosome binding but I don't think it is considered as part of the ribosome. I'm checking with an expert.

I don't see it in Figure 1. of the Ramakrishnan paper, where do you see it?

ValWood commented 3 years ago

uniprot product calls it Peptidyl-tRNA hydrolase ICT1, mitochondrial I think the name might be just historical.

ValWood commented 3 years ago

Actually

"A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome." Richter R., Rorbach J., Pajak A., Smith P.M., Wessels H.J., Huynen M.A., Smeitink J.A., Lightowlers R.N., Chrzanowska-Lightowlers Z.M. EMBO J. 29:1116-1125(2010)

...but this contrast with information I received recently. I'll get back to you.

pgaudet commented 3 years ago

Its Fig 3B. The gene is labeled ICT1.

image
ValWood commented 3 years ago

OK, I won't filter this. If things change (there is an upcoming paper),I will reopen.

pgaudet commented 3 years ago

Great, thanks.