protein phosphatase type 7 activity

[gocentral]

Suggested new term : protein phosphatase type 7 activity Synonyms: Protein phosphatase with EF calcium-binding domain (PPEF)

Definition: Catalysis of the reaction: a phosphoprotein + H2O = a protein + phosphate. A protein serine/threonine phosphatase containing EF-hand calcuim binding domains

References

UniProt:PPE1_HUMAN O14829 UniProt:PPE1_MOUSE O35655 UniProt:PPE2_HUMAN O14830 UniProt:PPE2_MOUSE O35385

PubMed ID: 9430683 Huang X., Honkanen R.E.; "Molecular cloning, expression, and characterization of a novel human serine/threonine protein phosphatase, PP7, that is homologous to Drosophila retinal degeneration C gene product (rdgC)."; J. Biol. Chem. 273:1462-1468(1998).

PubMed ID : 9326663 Sherman,P.M., Sun,H., Macke,J.P., Williams,J., Smallwood,P.M., Nathans,J., Identification and characterization of a conserved family of protein serine/threonine phosphatases homologous to Drosophila retinal degeneration C. (1997) Proc. Natl. Acad. Sci. U.S.A. 94:11639-11644

Reported by: kw_phos

Original Ticket: "geneontology/ontology-requests/1459":https://sourceforge.net/p/geneontology/ontology-requests/1459

[gocentral]

Logged In: YES user_id=473796

Hi Katy,

Do you have a more functional definition for this term? Can it be distinguished from the other protein phosphatases on the basis of its activity or is it structural differences that differentiate them? The same applies to the other protein phosphatase term you've requested.

Original comment by: girlwithglasses

[gocentral]

Logged In: YES user_id=951704

Hi Amelia,

I tried to come up with definitions that were similar to the ones already in GO for the rest of the serine/threonine phosphatases (see below). The reaction mechanisms (and therefore the function in a broad sense) are the same for each s/t phosphatase type. The differences in structure determine substrate specificity allowing different phosphatases to act in different pathways/systems. The structural features in my definitions, EF hand in PP7 and TPR in PP5, distinguish those phosphatases from any other type. There is also a protein phosphatase type 6. It is thought to have a close evolutional relationship with PP2A. I didn't suggest a definition for this phosphatase as I was unable to find and functional (or structural) definition good enough to distinguish it from PP2A.

I hope this helps,

Katy

GO:0030357 protein phosphatase type 2B activity - Catalysis of the reaction: a phosphoprotein + n H2O = a protein + n phosphate. GO:0015071 protein phosphatase type 2c activity - Catalysis of the reaction: a phosphoprotein + H2O = a protein + phosphate.

** GO:0000163 protein phosphatase type 1 activity - Catalysis of the reaction: a phosphoprotein + H2O = a protein + phosphate. An ATP, magnesium-dependent protein serine/threonine phosphatase (AMD phosphatase) that consists of a catalytic subunit of 38 kDa and a modulator subunit of 23 kDa, which is subject to phosphorylation, thus activating the phosphatase

** GO:0000158 protein phosphatase type 2A activity -Catalysis of the reaction: a phosphoprotein + H2O = a protein + phosphate. A protein serine/threonine phosphatase that is polycation-stimulated (PCS), being directly stimulated by protamine, polylysine, or histone H1; it constitutes a subclass of several enzymes activated by different histones and polylysine.

Original comment by: kw_phos

[gocentral]

Logged In: YES user_id=451873

Hi Katy - yes, those child terms of protein phosphatase have bad definitions - it looks like most of them aren't valid GO functions, they're quite old terms. Amelia is slowly working her way through the function ontology removing these duplicate functions, so these will be up for review in due course. For now, I'd suggest annotating directly to 'serine/threonine protein phosphatase' and perhaps 'calcium binding'? We don't include protein structure info in GO, so we can't add this as a new term I'm afraid.

Original comment by: jl242

[gocentral]

• milestone: --> TermGenie
• assigned_to: nobody --> jl242
• status: open --> closed-rejected

Original comment by: jl242