Closed pgaudet closed 6 years ago
Definition as agreed with Colin Logie from GREEKC: Integrator of signals between enhancers and the RNA polymerase I general transcriptional machinery that assembles at promoters as the preinitiation complex (PIC) to control transcription initiation. General cofactors are required by most genes for efficient transcription initiation.
Discussed at Dec 18 ontology editors's call: suggestion to make the definition more 'function-like':
How about: Bridging promoter cis-regulatory elements with the RNA polymerase I general transcriptional machinery that assembles at promoters as the preinitiation complex (PIC) to control transcription initiation. General cofactors are required by most genes for efficient transcription initiation. The most common general cofactor for RNA polymerase II is the mediator complex.
@krchristie @tberardini @thomaspd @ukemi @hdrabkin
Hoes does that sound ?
Thanks, Pascale
I am concerned that use of the word "general transcription factor" in the context of RNAP I is artificial and will be confusing since I have never seen the word "general transcription factor" used for anything other than RNAP II. Unless the literature has changed significantly, and the word "general" is now used in the literature for RNAP I, I am against artificially applying this word to RNAP I.
I think that trying to standardize use of the word "general transcription factor" in this way is similar to what we were recently talking about with respect to "biogenesis" where Midori tried to standardize "biogenesis" versus "assembly", but in retrospect, it turned out to be confusing because the GO use of the words were inconsistent with usage in the literature.
Hi @krchristie
I think this terminology is pretty standard, see https://en.wikipedia.org/wiki/General_transcription_factor PMID:25693126 PMID: 27816209
Would it be clearer if we phrased it as 'general transcription initiation factor activity'?
Thanks, Pascale
This is the name of the protein (at least as annotated in UniProt for human) - http://www.uniprot.org/uniprot/Q9NYV6 RNA polymerase I-specific transcription initiation factor RRN3
Thanks, Pascale
Both of the PMIDs you cite are specific to RNAP II:
The Wiki page you cite is also almost entirely about RNAP II, with a brief mention of bacteria, and while it is true that the [unknown] wiki author refers to sigma as a GTF, I have never seen any bacterial transcription researchers refer to sigma as a GTF.
The name of the RNAP I transcription factor that you are trying to rename also does NOT call it a "general transcription factor".
I do not think any of the things you have cited support the contention that the use of the phrase "general transcription factor" is appropriate for anything except RNAP II.
just call it "RNA polymerase I cofactor activity"?
or drop the gene name RRN3 off of UniProt's name for RRN3 and call it something like "RNA polymerase I-specific transcription initiation factor"
How about "RNA polymerase I promoter selection activity"? This is how we named the parent of sigma factors, so it would be consistent with that.
Thanks, Pascale
It doesn't seem like RRN3 is comparable to sigma factors since it doesn't seem to recognize the DNA itself, nor is this nomenclature used in the RNAP I literature, so misappropriating this bacterial terminology for RNAP I seems contrary to your goal of using term names more comprehensible for curators & users.
from intro of: Sadian Y, et al. Structural insights into transcription initiation by yeast RNA polymerase I. EMBO J. 2017 Sep 15;36(18):2698-2709. PMID: 28739580
For initiating rRNA synthesis, yeast Pol I uses a unique set of transcription factors: Upstream Activating Factor (UAF), TATA‐binding protein (TBP), Core Factor (CF), and Rrn3 (Keener et al, 1998).
Changed label: OLD name: transcription factor activity, core RNA polymerase I binding NEW name: RNA polymerase I transcription initiation factor
Changed definition: OLD def: "Interacting selectively and non-covalently with an RNA polymerase I (RNAP I) complex, typically composed of fourteen subunits, in order to modulate transcription. A protein binding transcription factor may or may not also interact with the template nucleic acid (either DNA or RNA) as well." [GOC:txnOH] NEW def: "An activity that contributes to transcription start site selection and transcription initiation of genes transcribed by RNA polymerase I. Factors required for RNA polymerase I transcription initiation include upstream activation factor (UAF), core factor (CF), TATA binding protein (TBP) and RRN3." [GOC:txnOH-2018, PMID:28340337, PMID:28842442]
Changed synonym types to BROAD As the Core Factor also binds the polymerase synonym: "core RNA polymerase I binding transcription factor activity" BROAD [] synonym: "transcription factor activity, core RNA polymerase I binding" BROAD []
Rename 'GO:0001181 transcription factor activity, core RNA polymerase I binding' -> general RNA polymerase I cofactor activity
and move under transcription cofactor activity.
Thanks, Pascale