glutamate-cysteine ligase regulator

[gocentral]

A new term request:

enzyme regulator activity ; GO:0030234 --%glutamate-cysteine ligase regulator activity ; GO:NEW

Modulates the activity of glutamate-cysteine ligase (GO:0004357), by lowering its sensitivity to inhibition by glutathione and by increasing its affinity for glutamate. PMID:12954617

Also, a new exact synonym for glutamate-cysteine ligase activity ; GO:0004357 ; EC:6.3.2.2: gamma-glutamylcysteine synthetase activity http://www.chem.qmul.ac.uk/iubmb/enzyme/EC6/3/2/2.htm

(needed for FBrf0167749)

I'll plan to add in on Wed 4th Aug.

thanks, Becky

Reported by: beckyfoulger

Original Ticket: "geneontology/ontology-requests/1902":https://sourceforge.net/p/geneontology/ontology-requests/1902

[gocentral]

Logged In: YES user_id=473796

Could you make this a process term instead of a function, since you've named a couple of methods of regulation?

Original comment by: girlwithglasses

[gocentral]

Logged In: YES user_id=482748

Can I make a process and a function...

glutamate-cysteine ligase regulator activity ; GO:NEW Modulates the activity of glutamate-cysteine ligase (GO:0004357).

regulation of enzyme activity ; GO:0050790 --%regulation of glutamate-cysteine ligase activity ; GO:NEW ----%negative regulation of glutamate-cysteine ligase activity ; GO:NEW ----%positive regulation of glutamate-cysteine ligase activity ; GO:NEW

regulation of glutamate-cysteine ligase activity ; GO:NEW Any process that modulates the activity of glutamate-cysteine ligase.

negative regulation of glutamate-cysteine ligase activity ; GO:NEW Any process that stops or reduces the activity of the enzyme nitric-oxide synthase.

positive regulation of glutamate-cysteine ligase activity ; GO:NEW Any process that activates or increases the activity of glutamate-cysteine ligase, typically by lowering its sensitivity to inhibition by glutathione and by increasing its affinity for glutamate. PMID:12954617

Original comment by: beckyfoulger

[gocentral]

Logged In: YES user_id=482748

my cut and paste seemed to die. Obviously I meant to type 'glutamate-cysteine ligase activity' in all the definitions.

Original comment by: beckyfoulger

[gocentral]

Logged In: YES user_id=473796

Do you know how the regulation actually works? Does it bind to glutamate-cysteine ligase or something else? I think regulating enzyme activity is really a process, and the function term should describe the steps/method of regulation (eg binding).

Original comment by: girlwithglasses

[gocentral]

Logged In: YES user_id=482748

ok- I can see that modulating the activity of EC:6.3.2.2 sounds more like a process. I was just making a term similar to the current ones under 'enzyme regulator activity ; GO:0030234'' to annotate one subunit of GCL. Are GO:0030234 and children up for obsoletion?

glutamate-cysteine ligase (GCL) is a heterodimer, consisting of a catalytic subunit and a regulatory/modifier subunit. According to PMID:12954617, the modifier subunit regulates catalytic activity by covalent and non-covalent interactions with the catalytic subunit.

I've already annotated to glutamate-cysteine ligase complex ; GO:0017109 (which should be moved from unlocalised to under cytoplasm- see PMID:12954617).

It doesn't seem correct to make a 'glutamate-cysteine ligase binding' term because the regulatory subunit is still part of GCL, just not the catalytic part. So for a function term, can I have:

enzyme binding --%glutamate-cysteine ligase catalytic subunit-binding ; GO:new ?? Interacting selectively with the catalytic subunit of glutamate-cysteine ligase.

?

Original comment by: beckyfoulger

[gocentral]

Logged In: YES user_id=473796

At the moment the enzyme regulator terms aren't up for obsoletion but they're on my list for obsoletion at some point in the future. Let's get 'transcription factor' out first. ;)

The subunit binding term sounds fine (but you don't need the hyphen between subunit and binding). If you could move the component term at the same time, that would be even better!

Original comment by: girlwithglasses

[gocentral]

Logged In: YES user_id=482748

1/ added the synonym gamma-glutamylcysteine synthetase activity to GO:0004357.

2/ Moved GO:0017109 to be a part_of child of cytoplasm.

3/ Added the following new terms:

glutamate-cysteine ligase catalytic subunit binding ; GO:0035226 regulation of glutamate-cysteine ligase activity ; GO:0035227 negative regulation of glutamate-cysteine ligase activity ; GO:0035228 positive regulation of glutamate-cysteine ligase activity ; GO:0035229

Have not created specific enzyme regulator terms.

Original comment by: beckyfoulger

[gocentral]

• status: open --> closed-fixed

Original comment by: beckyfoulger