Error in 'mitochondrial processing peptidase complex' (GO:0017087) definition.

[sjm41]

Current def: A protein complex that consists of a catalytic alpha subunit (alpha-MPP) and a regulatory beta subunit (beta-MPP), and catalyzes the release of N-terminal targeting peptides from precursor proteins imported into the mitochondrion. Source: BRENDA:3.4.24.64, GOC:mah, EC:3.4.24.64

I think the given functions of the alpha and beta subunits are the wrong way round.

See PMID: 12191769 for a review.

Also see descriptions on the UniProt entries for the human/yeast proteins: Human alpha subunit (PMPCA) - https://www.uniprot.org/uniprot/Q10713 Human beta subunit (PMPCB) - https://www.uniprot.org/uniprot/O75439 cerevisiae alpha subunit (MAS2) - https://www.uniprot.org/uniprot/P11914 cerevisiae beta subunit (MAS1) - https://www.uniprot.org/uniprot/P10507

[pgaudet]

I will also remove the EC and the BRENDA as those should be in MF (right @hdrabkin ??)

[pgaudet]

Interestingly we dont have a MF for EC:3.4.24.64. The closest term is 'metalloaminopeptidase activity'

Should I add the xref there ? @hdrabkin Although that might mean adding all sorts of EC xrefs for various peptidases that have the same activity, such as this list: https://enzyme.expasy.org/EC/3.4.24.-

[sjm41]

We used to - GO:0004240 (obsolete)

[pgaudet]

Right, because the activity is no different from any metalloprotease

[hdrabkin]

The EC should be for the MFs. The EC:3.4.24.64 doesn't have a RHEA, most likely because the substrates would be too generic (Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2).

[ValWood]

I used "metalloendopeptidase activity" because it's an internal bond, but oddly this isn't a descendant of GO:0070006 metalloaminopeptidase activity?

[ValWood]

I'll open a new ticket for that...