NEW term? acyl–coenzyme A diphosphatase

[ValWood]

Do we have a term for this activity?

FIT2 (FITM2) is an acyl–coenzyme A diphosphatase crucial for endoplasmic reticulum homeostasis | PMID: 32915949

[deustp01]

No, AFAIK, but there is a Rhea reaction

[alanbridge]

Hi Peter and Val,

hope you are doing well, why not try the parent reaction as a base for this term:

https://www.rhea-db.org/rhea/50044

an acyl-CoA + H2O = adenosine 3',5'-bisphosphate + an acyl-4'-phosphopantetheine + 2 H(+)

if we are missing Rhea annotations based on exptl data don't hesitate to drop UniProt a line too.

All the best, Alan

[hdrabkin]

Hi @ValWood Since in the paper, 'Oleoyl-CoA and arachidonoyl-CoA were the only detected fatty acyl-CoAs, and levels of both species were increased in FIT2-KO cells compared with WT cells (Fig. 3 E), consistent with both being substrates for the enzyme" I'm more inclined to make the more generic reaction suggested by @alanbridge Interestingly, the paper you cite is used by RHEA for the more specific term. What would you think is good parent term?

[pgaudet]

parent could be GO:0016462 pyrophosphatase activity?

[ValWood]

Interestingly, the paper you cite is used by RHEA for the more specific term.

I think it is the only paper, was a recent novel gene characterization in my unknown set ;)

[alanbridge]

Funnily enough (and from a completely different angle) we just designated ndx-8 in C. elegans for an update in UniProt (https://www.uniprot.org/uniprot/Q9NA25). It has this text annotation:

"Coenzyme A diphosphatase which mediates the cleavage of CoA into 3',5'-ADP and 4'-phosphopantetheine" but has no Rhea.

We also tagged the related protein NUDT15 in Arabidopsis (https://www.uniprot.org/uniprot/Q8GYB1), which has again no Rhea but this comment:

"Coenzyme A diphosphatase which mediates the cleavage of oxidized CoA. Can use malonyl-CoA, hexanoyl-CoA, lauroyl-CoA, myristoyl-CoA and palmitoyl-CoA as substrates, but not isobutyryl-CoA or propionyl-CoA".

Maybe one for PAINTing? Pinging @marcfeuermann !

[ValWood]

note to self SPBC543.08

[ValWood]

@hdrabkin any news here?

[hdrabkin]

Sorry for the delay

+id: GO:0106399 +name: acyl–coenzyme A diphosphatase activity

[ValWood]

@suzialeksander could you annotate FIT2 to this term? I will ISO to SGD:FIT2. I'm guessing UniProt will deal with the human annotation. Tagging @Antonialock if it isn't on anyone's radar already.

[ValWood]

Actually SGD seems to be YFT2 (FIT2 seems to be a synonym for yeast and humann)