NTR:phlorizin hydrolase activity

[lbreuza]

Please provide as much information as you can:

• Suggested term label: Phlorizin hydrolase activity

• Definition (free text) Catalysis of the reaction: phlorizin + H2O = beta-D-glucose + phloretin.

• Reference, in format PMID:####### PubMed=9762914

• Gene product name and ID to be annotated to this term LCT - UniProt/P09848

• Parent term(s) GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

• Children terms (if applicable) Should any existing terms that should be moved underneath this new proposed term?

• Synonyms (please specify, EXACT, BROAD, NARROW or RELATED)

• Cross-references

• For enzymes, please provide RHEA and/or EC numbers.

• Can also provide MetaCyc, KEGG, Wikipedia, and other links. RHEA:69639 (not yet public)

• Any other information

[pgaudet]

The reaction that corresponds to this already exists - if the EC in P09848 is correct (EC:3.2.1.62) - GO:0017042 glycosylceramidase activity

I will add 'phlorizin hydrolase activity' as a synonym.

OK ?

[pgaudet]

Actually the synonyms are already there - I wonder why you didn't find the term? Maybe P2GO only lists labels and not synonyms?

[pgaudet]

@amorgat @kaxelsen

How come EC:3.2.1.62 does not map to RHEA:69639 ?

[lbreuza]

I found that term, but phlorizin is not a Glycosyl-N-acylsphingosine.

[kaxelsen]

Exactly, phlorizin is a dihydrochalcone glucoside.

[pgaudet]

So EC should not have these terms as a synonyms? https://enzyme.expasy.org/EC/3.2.1.62

[kaxelsen]

You are right. This EC entry was created before it was settled the enzyme has two glycosyl hydrolase domains with each their activity. I'm going to submit an update very soon. The phlorizin hydrolase activity belongs to the EC 3.2.1.108 (lactase) domain.