tubercle

[gocentral]

Hi,

does anyone know something about "tubercle"? It is the location for the enzyme(s) 3.4.21.34 (plasma kallikrein, tissue kallikrein). It seems to be a small knob on the worm Schistosoma mansoni, but I can't see if this is a part of cell or an own cell. See http://memorias.ioc.fiocruz.br/983/4717.pdf.

Thanks, Marion

Reported by: brendamg

Original Ticket: "geneontology/ontology-requests/2363":https://sourceforge.net/p/geneontology/ontology-requests/2363

[gocentral]

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According to the dictionary it's a collection of cells:

tubercle <microbiology> Chronic inflammatory focus, a granuloma, caused by Mycobacterium tuberculosis.

granuloma <pathology> Chronic inflammatory lesion characterised by large numbers of cells of various types (macrophages, lymphocytes, fibroblasts, giant cells), some degrading and some repairing the tissues.

Original comment by: jl242

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According to the dictionary it's a collection of cells:

tubercle <microbiology> Chronic inflammatory focus, a granuloma, caused by Mycobacterium tuberculosis.

granuloma <pathology> Chronic inflammatory lesion characterised by large numbers of cells of various types (macrophages, lymphocytes, fibroblasts, giant cells), some degrading and some repairing the tissues.

Original comment by: jl242

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I think in the organism Schistosoma mansoni, there is another meaning of the the term "tubercle". Something like an epidermal outgrowth...

Original comment by: brendamg

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Have contacted the parasite group and Sanger to see if they can help.

Original comment by: jl242

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[from R.A. Wilson, Univeristy of York]

This one is complicated.

The adult male dorsal surface is covered in small bosses called tubercles that provide purchase against the blood vessel wall when the worm is crawling up the mesenteric veins carrying the female in its ventral groove. Each tubercle has a thin (2-3 micron) covering of the syncytial tegument. Enclosed entirely within the tegument cytoplasm of the tubercle are a series of spines, made of actin, which assist in gripping the blood vessel.

Now the complicated bit. The tegument is normally closely apposed to the underlying circular muscle layer, and separted from it only by an extracellular matrix of collagen fibres. The tubercle is the exception, where the tegument is lifted up by numerous extensions of parenchymal cells. The parenchymal cell bodies lie below the muscle layers and send out narrow extensions of cytoplasm which insinuate between the muscles to form the packing for the tubercle. (I could provide a diagram if this would help.) However, beware of someone saying that a protein or transcript is present in the dorsal tubercles - it could simply be a parenchymal (i.e. body packing protein) molecule.

Alan

Original comment by: jl242

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Hi Marion - presumably 3.4.21.34 is located in the cytoplasm part of the tubercle, which is actually part of the parenchymal cell bodies? Or does the source not specify?

Original comment by: jl242

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Original comment by: jl242

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Hi Jane - the source is PMID: 10340330. Immunolocalization of the native protein "shows a positive signal in the dorsal tubercles covering the cuticle in male worms and in parenchyma in both sexes. The signal was absent from muscle layers and tegument." So I think cytoplasm should not be wrong. In addition we will keep tubercle as a tissue. Thanks for the definitions.

Original comment by: brendamg

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Great. I'll close this then.

Original comment by: jl242

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• status: open --> closed-works-for-me

Original comment by: jl242