GO:0018741 alkyl sulfatase activity def restricts to dodecyl (C12H25) chains

[suzialeksander]

• GO term ID and label for which you request a definition update GO:0018741 alkyl sulfatase activity (https://www.ebi.ac.uk/QuickGO/services/ontology/go/terms/GO:0018741/complete)

• New proposed definition (genus-differentia pattern, plus additional information if needed) New definition should be something generic, not referring to dodecyl: Catalysis of the reaction: an alkyl sulfate ester + H2O <=> a primary alcohol + H(+) + sulfate

• Reference(s) https://enzyme.expasy.org/EC/3.1.6.21 PMID: 28720494 https://doi.org/10.1016/j.bbrc.2017.07.092 -> source of attached image that shows alkyl sulfate hydrolysis on something other than C12

• Additional information

• the cross reference UM-BBD_reactionID r0602 is quite narrow

[hdrabkin]

current ec xref EC:3.1.6.-. maps to Hydrolases Acting on ester bonds Sulfuric ester hydrolases Even 3.1.6.21 seems more specific (linear primary-alkylsulfatase.

[hdrabkin]

Added EC and RHEA xrefs and changed def as requested/ RHEA and EC specifically say linear primary-alkylsufatase, which is more specific, so making those narrowMarch exrefs

DOne

[pgaudet]

Based on the EXP 2 annotations, I think it would be safe to align with RHEA and EC and make this a 'linear linear primary alkylsulfatase'?

[hdrabkin]

Just checked that there are two other ECs for non-linear substrates. So, if we remove narrow match, then the name and def should be changed to reflect the liniear chain substrate. Leaving it 'alkyl' is ambiguous. EC 3.1.6.19 (R)-specific secondary-alkylsulfatase (type III) EC 3.1.6.22 branched primary-alkylsulfatase.

So would change GO:0018741 term name to linear primary-alkylsulfatase activity with def like a primary linear alkyl sulfate ester + H2O= primary alcohol + H(+) + sulfate (ie, as it was), or the RHEA def (basically the same def).

I checked the SGD paper and they used SDS (linear) when testing for growth.

[hdrabkin]

Just checked that there are two other ECs for non-linear substrates. So, if we remove narrow match, then the name and def should be changed to reflect the liniear chain substrate. Leaving it 'alkyl' is ambiguous. EC 3.1.6.19 (R)-specific secondary-alkylsulfatase (type III) EC 3.1.6.22 branched primary-alkylsulfatase.

So would change GO:0018741 term name to linear primary-alkylsulfatase activity with def like a primary linear alkyl sulfate ester + H2O= primary alcohol + H(+) + sulfate (ie, as it was), or the RHEA def (basically the same def).

I checked the SGD paper and they used SDS (linear) when testing for growth.