kaxelsen
commented
1 year ago
- The EC:2.7.7.19 xref on polynucleotide adenylyltransferase activity (GO:0004652) also specifies RNA in the 'reaction' (matching RHEA:11332), though the second comment on the EC entry (in bold below) specifies DNA - I suspect this is a typo for "RNA" @kaxelsen?
I think you are right. It is incredible nobody has noticed before, the entry was created in 1965 and have never been revised since. The only changes I can see are a couple of new papers added in the early seventies. I will correct the typo.
pgaudet
Terms to be merged: Provide GO ID and term label polynucleotide adenylyltransferase activity (GO:0004652) RNA adenylyltransferase activity (GO:1990817)
polynucleotide adenylyltransferase activity (GO:0004652) has a single is_a child term, RNA adenylyltransferase activity (GO:1990817) - there is no "DNA adenylyltransferase activity" sister GO term
The RHEA:11332 xref on polynucleotide adenylyltransferase activity (GO:0004652) specifies RNA, so should be on RNA adenylyltransferase activity (GO:1990817)
The EC:2.7.7.19 xref on polynucleotide adenylyltransferase activity (GO:0004652) also specifies RNA in the 'reaction' (matching RHEA:11332), though the second comment on the EC entry (in bold below) specifies DNA - I suspect this is a typo for "RNA" @kaxelsen?
=> Seems like GO:0004652 and GO:1990817 could be merged with the final term name being "poly(A) RNA polymerase activity", retaining the definition on GO:1990817
id: GO:0004652 name: polynucleotide adenylyltransferase activity def: "Catalysis of the template-independent extension of the 3'- end of an RNA or DNA strand by addition of one adenosine molecule at a time. Cannot initiate a chain 'de novo'. The primer, depending on the source of the enzyme, may be an RNA or DNA fragment, or oligo(A) bearing a 3'-OH terminal group." [EC:2.7.7.19] xref: EC:2.7.7.19 xref: MetaCyc:POLYNUCLEOTIDE-ADENYLYLTRANSFERASE-RXN xref: Reactome:R-HSA-72185 "mRNA polyadenylation" xref: RHEA:11332 is_a: GO:0070566 ! adenylyltransferase activity
id: GO:1990817 name: RNA adenylyltransferase activity namespace: molecular_function def: "Catalysis of the template-independent extension of the 3'- end of an RNA strand by addition of one adenosine molecule at a time. Cannot initiate a chain 'de novo'. The primer, depending on the source of the enzyme, may be an RNA, or oligo(A) bearing a 3'-OH terminal group." [GOC:vw] is_a: GO:0004652 ! polynucleotide adenylyltransferase activity
https://enzyme.expasy.org/EC/2.7.7.19 Name: polynucleotide adenylyltransferase. Alternative Name(s): NTP polymerase, poly(A) polymerase, RNA adenylating enzyme. Reaction: ATP + RNA(n) <=> diphosphate + RNA(n)-3'-adenine ribonucleotide Comment(s): Also acts slowly with CTP. Catalyzes template-independent extension of the 3'-end of a DNA strand by one nucleotide at a time. Cannot initiate a chain de novo. The primer, depending on the source of the enzyme, may be an RNA or DNA fragment or oligo(A) bearing a 3'-OH terminal group.
https://www.rhea-db.org/rhea/11332 ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide