NTR: tRNA methyltransferase + activator activity

[Antonialock]

Hello, this paper came up in my log files so I took a look at the paper, so I'm requesting these terms to the best of my knowledge. I see there is some ongoing refactoring in these activities.

• Suggested term label:

  1. tRNA (guanine(6)-N2)-methyltransferase activity
  2. tRNA (guanine(7)-N2)-methyltransferase activity
  3. tRNA (guanine(6)-N2)-methyltransferase activator activity
  4. tRNA (guanine(7)-N2)-methyltransferase activator activity

• Definition (free text) standard

• Reference, in format PMID:####### PMID:34669960 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599901

THUMPD3 is responsible for tRNA: m2G6 formation in human cells. However, THUMPD3 alone could not modify tRNAs. Instead, multifunctional methyltransferase subunit TRM112-like protein (TRMT112) interacts with THUMPD3 to activate its methyltransferase activity.

THUMPD3–TRMT112 could methylate all the 26 tested G6-containing human cytoplasmic tRNAs by recognizing the characteristic 3′-CCA of mature tRNAs. We also showed that m2G7 of tRNATrp was introduced by THUMPD3–TRMT112.

• Gene product name and ID to be annotated to this term 1 and 2 for THUMPD3 3 and 4 for TRMT112

• Parent term(s)

standard

[pgaudet]

For the methyltransferases:

Reaction 1 corresponds to

• EC 2.1.1.256 tRNA (guanine(6)-N(2))-methyltransferase and RHEA:51116
• term label "tRNA (guanine(6)-N(2))-methyltransferase activity"
• parent: tRNA (guanine) methyltransferase activity

I dont find an EC or a RHEA for Reaction 2; have these been requested?

For the activators: We will not create an activator for each methyltransferase; @Antonialock can you please use tRNA methyltransferase activator activity? and 'has input' the enzyme regulated?

Thanks, Pascale

[Antonialock]

ah thanks, I did not see guanine(6)-N(2))-methyltransferase activity I did not request the rhea @kaxelsen

activators: that makes sense, thanks!

[raymond91125]

[Term] id: GO:0160117 name: tRNA (guanine(6)-N2)-methyltransferase activity namespace: molecular_function def: "Catalysis of the reaction: guanosine(6) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)-methylguanosine(6) in tRNA + S-adenosyl-L-homocysteine." [EC:2.1.1.256, PMID:34669960] synonym: "m(2)G6 methyltransferase" EXACT [] synonym: "methyltransferase Trm14" EXACT [] xref: EC:2.1.1.256 xref: RHEA:51116 is_a: GO:0008170 ! N-methyltransferase activity is_a: GO:0016423 ! tRNA (guanine) methyltransferase activity property_value: term_tracker_item "https://github.com/geneontology/go-ontology/issues/26306" xsd:anyURI created_by: rynl creation_date: 2023-10-26T23:14:33Z

[Term] id: GO:0160118 name: tRNA (guanine(7)-N2)-methyltransferase activity namespace: molecular_function def: "Catalysis of the reaction: guanosine(7) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)-methylguanosine(7) in tRNA + S-adenosyl-L-homocysteine." [PMID:34669960] is_a: GO:0008170 ! N-methyltransferase activity is_a: GO:0016423 ! tRNA (guanine) methyltransferase activity property_value: term_tracker_item "https://github.com/geneontology/go-ontology/issues/26306" xsd:anyURI created_by: rynl creation_date: 2023-10-26T23:24:47Z