Taxon constraint: GO:0015039 | NADPH-adrenodoxin reductase activity

[ValWood]

Please provide as much information as you can:

• GO term ID label:

These annotations are still present, I will raise a taxon restriction for GO:0015039 | NADPH-adrenodoxin reductase activity I need to double check species distribution. Andredoxin seems to be present in some fungi, but not pombe or cerevisiae.

• Request to add a taxon constraint:

• • only in taxon:
• • never in taxon:

• Request to remove a taxon constraint: Please specify

• Supporting evidence if available (e.g PMID):

[ValWood]

From a quick glance, adrenodoxin reductase seems to be a gene-product specific name for ferridoxin-reductase?

NADPH-adrenodoxin reductase activity also has a comment: Note that this term specifically refers to the reaction proceeding in the direction shown; under physiological conditions adrenodoxin reduction by adrenodoxin reductase is coupled with electron transfer from AdR to P450, which catalyzes an irreversible monooxygenation reaction. This term should therefore be used to annotate gene products that catalyze the reduction of oxidized adrenodoxin; also consider annotating to the molecular function term 'ferredoxin-NADP+ reductase activity; GO:0004324'.

I haven't yet found out exactly which cytochrome adrenodoxin refers to and if yeast have it. I keep finding adrenodoxin == ferridoxin...

[ValWood]

@deustp01 do you know anything about NADPH-adrenodoxin reductase activity

[deustp01]

My vague overview understanding is that many enzymes that mediate oxidation-reductiion reactions on small-molecule substrates (sterols and drugs are popular ones) have bound cofactors like iron-sulfur clusters and others I know even less about, that are reduced-oxidized in the course of the reaction. That of course inactivates the enzyme. For reactivation it needs to undergo a reaction in which that bound cofactor is re-oxidized or re-reduced. Adrenodoxins, which also definitely exist in mammals - see this UniProt entry for a human one), and examine its first linked RHEA reaction - can mediate this cofactor regeneration for iron-sulfur clusters.

A confusing factor for me is that textbook diagrams of oxidation-reduction reactions often represent iron-sulfer clusters and FAD/FMN as entities in their own right, not as cofactor components of a protein or protein complex.

On this view, I guess adrenodoxin activity is required for the textbook oxidation of a sterol or a drug, but indirectly, as the agent responsible for maintaining the gene product that does the actual chemistry in its active state.

Beyond that, I am also still struggling to understand how these things work.

[ValWood]

Since adrenodoxin appears to be a speicific type of ferridoxin, found in adrenal cells, I will add a taxon restriction for fungi

[pgaudet]

There already is a TC - did you forget to link the ticket when you did the work?

Thanks, Pascale

[ValWood]

I probably did it when I was trying to edit taxon constraints in VSC (which meant they got pulled into a pull request , but might not of had a term tracker item) I now do these in the web editor again, which is OK, and easier to keep track of I think my issue was that I didn't realise there was a final tab in the file!

Anyway closing this. I still think this should probably be is_a ferredoxin but I'll leave that to somebody who knows more.....