pgaudet
commented
4 months ago Noting that the GO reaction talks about a 2nt overhang "Catalysis of the endonucleolytic cleavage of RNA with 5'-phosphomonoesters and 3'-OH termini; makes two staggered cuts in both strands of dsRNA, leaving a 3' overhang of 2 nt.", not mentioned by EC https://enzyme.expasy.org/EC/3.1.26.3
id: GO:0016443 name: bidentate ribonuclease III activity namespace: molecular_function def: "Catalysis of the digestion of double-stranded RNAs into 20 to 30-nucleotide products. These products typically associate to the RNA-induced silencing complex and serve as guide RNAs for posttranslational RNA interference." [PMID:15242644] synonym: "bidentate RNase III activity" EXACT [] xref: EC:3.1.26.- is_a: GO:0004525 ! ribonuclease III activity
GO:0016891 name: RNA endonuclease activity, producing 5'-phosphomonoesters def: "Catalysis of the hydrolysis of ester linkages within ribonucleic acids by creating internal breaks to yield 5'-phosphomonoesters." [GOC:ai] xref: EC:3.1.26.-
id: GO:0004525 name: ribonuclease III activity namespace: molecular_function def: "Catalysis of the endonucleolytic cleavage of RNA with 5'-phosphomonoesters and 3'-OH termini; makes two staggered cuts in both strands of dsRNA, leaving a 3' overhang of 2 nt." [PMID:11157775, PMID:15242644] synonym: "pre-mRNA 3'-end processing endonuclease" NARROW [] synonym: "ribonuclease 3 activity" RELATED [EC:3.1.26.3] synonym: "RNase III activity" RELATED [EC:3.1.26.3] xref: EC:3.1.26.3 xref: MetaCyc:3.1.26.3-RXN is_a: GO:0016891 ! RNA endonuclease activity, producing 5'-phosphomonoesters is_a: GO:0032296 ! double-stranded RNA-specific ribonuclease activity