gocentral
commented
17 years ago Logged In: YES user_id=436423 Originator: NO
added GO:0033263
I kept the CORVET def, and added a bit to define "endosomal tethering complex"; made it is_a endosomal part.
m
Original comment by: mah11
From Selina Dwight:
Based on PubMed paper ID: 17488625
Here's a stab at a definition and parentage. I used the HOPS definition as framework
1) Definition: A multimeric protein complex that is an endosomal tethering complex (CORVET = class C core vacuole/endosome tethering) involved in endo-lysosomal biogenesis and required for transport between endosome and vacuole. The Saccharomyces cerevisiae complex contains Vps8p, Vps3p, Pep5p, Vps16p, Pep3p, and Vps33p.
(not sure whether CORVET definition should be in there or not...)
I searched PubMed for CORVET and didn't find anything else...
2) Parentage Here are things about cellular location within the paper:
In Summary: and a novel endosomal tethering (CORVET) complex
In Results: As shown in Figure 2B, Vps3 is found in a 700 kDa complex together with Vps8, Vps11, Vps16, Vps18, and Vps33. We also observe Vps3 in a second peak at around 120 kDa. To determine if Vps3 dissociated partially from the Vps8-class C complex during the chromatography step, we used Vps8 as the bait in our purification (Figure 2C). Strikingly, the purification yielded a very similar picture, with Vps3 being present in the Vps8-class C complex and as a putative monomer. Vps3 must have been in a complex with Vps8 initially to be present on the gel filtration column and most likely dissociated partially from the complex during the column run, probably due to its distinct binding characteristics to the other subunits. This observation is underscored by the equimolar amounts of Vps3 and the other subunits. We therefore conclude that Vps3 is a subunit of a novel endosomal complex. Given that the complex contains the class C tethering proteins, functions at the endosome, and is required for transport between endosome and vacuole (see below), we term it CORVET complex (class C core vacuole/endosome tethering).
Reported by: krchristie
Original Ticket: "geneontology/ontology-requests/4208":https://sourceforge.net/p/geneontology/ontology-requests/4208