U4/U6 x U5 snRNP

[gocentral]

Could U4/U6 x U5 snRNP be added as a child of the term small nuclear ribonucleoprotein complex[30532]. Some proteins are components of U4/U6.U5 snRNP but not of U-6 or U5-specific snRNPs.

Justification:PMID: 11720284 Biochemical and genetic analyses of the U5, U6, and U4/ U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae.

We have purified the yeast U5 and U6 pre-mRNA splicing small nuclear ribonucleoproteins (snRNPs) by affinity chromatography and analyzed the associated polypeptides by mass spectrometry. The yeast U5 snRNP is composed of the two variants of U5 snRNA, six U5-specific proteins and the 7 proteins of the canonical Sm core. The U6 snRNP is composed of the U6 snRNA, Prp24, and the 7 Sm- Like (LSM) proteins. Surprisingly, the yeast DEAD-box helicase-like protein Prp28 is stably associated with the U5 snRNP, yet is absent from the purified U4/U6 x U5 snRNP. A novel yeast U5 and four novel yeast U4/U6 x U5 snRNP polypeptides were characterized by genetic and biochemical means to demonstrate their involvementin the pre-mRNA splicing reaction. We also show that, unlike the human tri-snRNP, the yeast tri-snRNP dissociated upon addition of ATP or dATP.

Reported by: lmatthews

Original Ticket: "geneontology/ontology-requests/477":https://sourceforge.net/p/geneontology/ontology-requests/477

[gocentral]

Logged In: YES user_id=473796

Term added, GO:0046540. Name changed to U4/U6 x U5 tri-snRNP complex

Original comment by: girlwithglasses

[gocentral]

• milestone: --> TermGenie
• assigned_to: nobody --> girlwithglasses
• status: open --> closed-accepted

Original comment by: girlwithglasses