the same? DNA methyltransferases

[gocentral]

GO:0003886 DNA (cytosine-5-)-methyltransferase activity

GO:0008326 site-specific DNA-methyltransferase (cytosine-specific) activity

Both have the definition:

Catalysis of the reaction: S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA 5- methylcytosine.

Reported by: tberardini

Original Ticket: "geneontology/ontology-requests/928":https://sourceforge.net/p/geneontology/ontology-requests/928

[gocentral]

• milestone: --> TermGenie
• assigned_to: nobody --> gomidori

Original comment by: mah11

[gocentral]

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It turns out to be a bit more complicated than I thought when I first saw this entry! GO:0008326 refers to the methylases that form part of restriction-modification systems, so the 'site-specific' bit refers to the same sort of recognition site as for restriction endonucleases.

I'm not sure whether GO:0003886 is supposed to be non-site-specific, but I didn't see anything about site specificity in the relevant Enzme Nomenclature entry (it's EC 2.1.1.37). I suspect it may be intended to mean the activity that causes methylation in CpG islands; if so, the terms are different. I'll email the list to see if anyone knows or has an opinion.

In any case, I'll modify the definition of 8326 to mention site specificity.

m

Original comment by: mah11

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Thanks for the clarification, Midori.

Tanya

Original comment by: tberardini

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Michael has confirmed that GO:0003886 is the activity that catalyzes CpG methylation, so I'm adding a comment. That and the change to the def of GO:0008326 should make the distinction between the two terms clearer.

m

Original comment by: mah11

[gocentral]

• status: open --> closed-fixed

Original comment by: mah11