NT: acyl-glucuronidase activity

[gocentral]

I would like to annotate ABHD10 (Q9NUJ1) from PMID:22294686 to

NT: acyl-glucuronidase activity

as a sibling of GO:0004566 beta-glucuronidase activity

I have asked ExPASy to take a look at the paper too, and see if they can create an EC number for this. Will add the EC number here when they get back to me.

Thanks, Varsha

Reported by: vkhodiyar

Original Ticket: geneontology/ontology-requests/9450

[gocentral]

Hi Varsha,

Do you have any idea what the product of the reaction is? Trying to work out what to put on the rhs of the reaction:

Catalysis of the reaction: acyl-glucuronide + H20 = ? + ?

Thanks, Becky

Original comment by: rebeccafoulger

[gocentral]

• assigned_to: nobody --> rfoulger

Original comment by: rebeccafoulger

[gocentral]

Sorry Becky I don't know, I am hoping that the ExPASy defintion will help us with this one.

Original comment by: vkhodiyar

[gocentral]

I wrote to the authors, and they say: In general, glucuronide (including acyl-glucuronide) is hydrolyzed to aglycone and glucuronic acid. In the case of acyl-glucuronide hydrolysis, aglycone is limited to the forms containing carboxylic acid.

I could add it in as a process, but it would be a shame to loose the enzyme info. So I'll add it in as a catalytic activity, and add a note that the formula may be incomplete.

Becky

Original comment by: rebeccafoulger

[gocentral]

That's great, thanks so much Becky.

Original comment by: vkhodiyar

[gocentral]

Hi Becky, I have just had this email from ExpASy:

Dear Varsha,

Thank you for your mail. The problem with mycophenolic acid acyl glucuronide (AcMPAG) is that it is not a natural occurring compound, and these are normally not added to the EC list. So it is very unlikely that an EC number will be created for this activity.

I will ask the other members of the nomenclature committee what they think about this case, but do not expect them to add this activity to the EC list.

On behalf of the UniProtKB/Swiss-Prot team,

Regards,

Kristian Axelsen

does this affect the GO term you are creating?

Varsha

Original comment by: vkhodiyar

[gocentral]

Hi Varsha,

Nope (not for process anyway)- for that reason I was going to keep the GO terms broad:

glucuronate metabolic process ; GO:0019585 --[isa]deglucorination ; GO:NEW ----[isa]acyl-deglucorination ; GO:NEW --[isa]cellular glucuronidation ; GO:0052695 ----[isa]acyl glucuronidation ; GO:NEW

The function is trickier. From a re-read of the paper, and similar papers, the original glucuronidation reaction is bidirectional. But ABHD10 is an esterase that catalyses the reverse (deglucorinidation) reaction (not sure it catalyses the glucuronidation).

I'm thinking of creating a bidirectional term: acyl-glucuronidase activity ; GO:NEW Catalysis of the reaction: an acyl--glucuronoside + H2O = an alcohol + D-glucuronate.

Then you can use the process terms to capture that it's removing the glucuronic-acid group.

Becky

Original comment by: rebeccafoulger

[gocentral]

Added: deglucuronidation ; GO:0036234 acyl deglucuronidation ; GO:0036235 acyl glucuronidation ; GO:0036236 acyl-glucuronidase activity ; GO:0036237

Happy curating, Becky

Original comment by: rebeccafoulger

[gocentral]

• status: open --> closed-works-for-me

Original comment by: rebeccafoulger

[gocentral]

Great, thank you!

Original comment by: vkhodiyar

[gocentral]

• status: closed-works-for-me --> open-works-for-me

Original comment by: vkhodiyar

[gocentral]

• status: open-works-for-me --> closed-works-for-me

Original comment by: rebeccafoulger