genome / docker-basespace_chromoseq

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Issues in Dockerfile #34

Open Sanaz01 opened 2 weeks ago

Sanaz01 commented 2 weeks ago

Hello, I am using docker image mgibio/chromoseq:v1.1 and facing the following issue in the task annotate_svs:

[M::mm_idx_gen::87.450*0.98] collected minimizers
[M::mm_idx_gen::118.619*0.98] sorted minimizers
[M::main::118.619*0.98] loaded/built the index for 194 target sequence(s)
[M::mm_mapopt_update::118.619*0.98] mid_occ = 1000
[M::mm_idx_stat] kmer size: 21; skip: 11; is_hpc: 0; #seq: 194
[M::mm_idx_stat::122.168*0.98] distinct minimizers: 381286576 (95.36% are singletons); average occurrences: 1.293; average spacing: 6.289; total length: 3099750718
[M::worker_pipeline::122.604*0.98] mapped 350 sequences
[M::main] Version: 2.17-r974-dirty
[M::main] CMD: /usr/local/bin/minimap2 -N 50 -p 0.5 --mask-level 0.8 -ax sr /cromwell-executions/ChromoSeq/bb88b56d-61ca-43ab-a5d3-86d5882dafe6/call-annotate_svs/shard-0/inputs/1314053308/Gencode_GRCh38.primary_assembly.genome.fa /tmp/input.fq
[M::main] Real time: 122.807 sec; CPU: 120.708 sec; Peak RSS: 11.343 GB
install_driver(mysql) failed: Can't locate DBD/mysql.pm in @INC (you may need to install the DBD::mysql module) (@INC contains: /opt/vep/ensembl-vep/modules /opt/vep/ensembl-vep /opt/lib/perl/VEP/Plugins /etc/perl /usr/local/lib/x86_64-linux-gnu/perl/5.22.1 /usr/local/share/perl/5.22.1 /usr/lib/x86_64-linux-gnu/perl5/5.22 /usr/share/perl5 /usr/lib/x86_64-linux-gnu/perl/5.22 /usr/share/perl/5.22 /usr/local/lib/site_perl /usr/lib/x86_64-linux-gnu/perl-base .) at (eval 34) line 3.
Perhaps the DBD::mysql perl module hasn't been fully installed,
or perhaps the capitalisation of 'mysql' isn't right.
Available drivers: DBM, ExampleP, File, Gofer, Mem, Proxy, Sponge.
 at /opt/vep/ensembl-vep/Bio/EnsEMBL/Registry.pm line 1769.

Any suggestions on how to resolve it?