geraldinepascal / FROGS

FROGS is a galaxy/CLI workflow designed to produce an OTUs count matrix from high depth sequencing amplicon data.
GNU General Public License v3.0
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FROGS 3.0.0 install - ITSx 1.0.11 install #37

Closed Toliman06 closed 5 years ago

Toliman06 commented 5 years ago

Hi (again...),

I'm currently installing ITSx 1.0.11 and I have some problem:

At this step, I have an error message:

Step 30/30 : RUN cd $LIBEXEC ; rm ITSx_db/HMMs/.h3 && for hmm in ls ITSx_db/HMMs/*.hmm ; do hmmpress $hmm; done ---> Running in f8bbbfe977d2 Working... done. Pressed and indexed 88 HMMs (88 names). Models pressed into binary file: ITSx_db/HMMs/A.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/A.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/A.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/A.hmm.h3p Working... done. Pressed and indexed 15 HMMs (15 names). Models pressed into binary file: ITSx_db/HMMs/B.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/B.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/B.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/B.hmm.h3p Working... done. Pressed and indexed 20 HMMs (20 names). Models pressed into binary file: ITSx_db/HMMs/C.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/C.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/C.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/C.hmm.h3p Working... done. Pressed and indexed 25 HMMs (25 names). Models pressed into binary file: ITSx_db/HMMs/D.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/D.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/D.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/D.hmm.h3p Working... done. Pressed and indexed 14 HMMs (14 names). Models pressed into binary file: ITSx_db/HMMs/E.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/E.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/E.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/E.hmm.h3p Working... done. Pressed and indexed 421 HMMs (421 names). Models pressed into binary file: ITSx_db/HMMs/F.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/F.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/F.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/F.hmm.h3p Working... done. Pressed and indexed 54 HMMs (54 names). Models pressed into binary file: ITSx_db/HMMs/G.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/G.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/G.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/G.hmm.h3p Working... done. Pressed and indexed 24 HMMs (24 names). Models pressed into binary file: ITSx_db/HMMs/H.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/H.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/H.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/H.hmm.h3p Working... done. Pressed and indexed 5 HMMs (5 names). Models pressed into binary file: ITSx_db/HMMs/I.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/I.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/I.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/I.hmm.h3p Working... done. Pressed and indexed 39 HMMs (39 names). Models pressed into binary file: ITSx_db/HMMs/L.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/L.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/L.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/L.hmm.h3p Working... done. Pressed and indexed 354 HMMs (354 names). Models pressed into binary file: ITSx_db/HMMs/M.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/M.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/M.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/M.hmm.h3p

Error: File format problem in trying to open HMM file ITSx_db/HMMs/N.hmm. �

Working... done. Pressed and indexed 71 HMMs (71 names). Models pressed into binary file: ITSx_db/HMMs/O.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/O.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/O.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/O.hmm.h3p Working... done. Pressed and indexed 10 HMMs (10 names). Models pressed into binary file: ITSx_db/HMMs/P.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/P.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/P.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/P.hmm.h3p Working... done. Pressed and indexed 7 HMMs (7 names). Models pressed into binary file: ITSx_db/HMMs/Q.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/Q.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/Q.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/Q.hmm.h3p Working... /bin/bash: line 1: 27 Segmentation fault (core dumped) hmmpress $hmm Working... done. Pressed and indexed 8 HMMs (8 names). Models pressed into binary file: ITSx_db/HMMs/S.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/S.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/S.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/S.hmm.h3p Working... done. Pressed and indexed 224 HMMs (224 names). Models pressed into binary file: ITSx_db/HMMs/T.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/T.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/T.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/T.hmm.h3p Working... done. Pressed and indexed 4 HMMs (4 names). Models pressed into binary file: ITSx_db/HMMs/U.hmm.h3m SSI index for binary model file: ITSx_db/HMMs/U.hmm.h3i Profiles (MSV part) pressed into: ITSx_db/HMMs/U.hmm.h3f Profiles (remainder) pressed into: ITSx_db/HMMs/U.hmm.h3p

I think this is due to a lack files for the "N": the N.hmm file size is 0 bytes and there is no other .h3 files in the ITSx tar.gz original downloaded file...

Is it a normal behaviour?

mariabernard commented 5 years ago

Hi,

I did not have any trouble installing ITSx, but yes if you miss some .hmm files it will not work. Please contact ISX support or try to download the file again.

In fact for FROGS (for now) it is not really a problem as we only use F.hmm* files (for fungi), and you seem to not have any problems with it.