gerlichlab / looptrace

Fork (from EMBL Gitlab) of the looptrace project: https://git.embl.de/grp-ellenberg/looptrace
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We should plot number of locus-specific FISH spots per probe/frame/timepoint #197

Closed vreuter closed 6 months ago

vreuter commented 6 months ago

@TLSteinacker

We have this functionality already elsewhere, but we should integrate it with the normal processing pipeline since it's often of interest for probe pool design / QC / etc.

Just do filtered spots since some of the filtration is purely technical, as we fit Gaussians to some extracted spot images that we'd ideally filter before the fitting.

vreuter commented 6 months ago

NB -- similar to #171, main difference Is no filtered/unfiltered distinction, and here we're interested in locus-specific FISH spots rather than ones from regional barcodes.

vreuter commented 6 months ago

Examples: locus-specific spot_counts bargraph locus-specific spot_counts filtered heatmap