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gerlichlab / looptrace

Fork (from EMBL Gitlab) of the looptrace project: https://git.embl.de/grp-ellenberg/looptrace
MIT License
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Only consider locus-specific spots from region(s) where they're expected #237

Closed vreuter closed 1 month ago

vreuter commented 5 months ago

In an experimental design, we know which locus-specific barcodes go with which regional barcodes, so to avoid using the same locus-specific spot multiple times when 2 regional barcodes' spots happen to be close and both overlap it, and to avoid exacerbating the potential problem of sample autofluorescence causing false locus-specific spots, we should use that a priori knowledge to ignore locus-specific spots for which (expected_ref_frame(frame) =!= ref_frame).

@TLSteinacker

vreuter commented 5 months ago

Depends on #81

vreuter commented 4 months ago

Only displaying these data (from regions where a locus spot is expected) is very important for UX when visualising locus-specific spots (#259 / #269 )