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looptrace
Fork (from EMBL Gitlab) of the looptrace project: https://git.embl.de/grp-ellenberg/looptrace
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Addition of analysis of non-FISH signal in regions defined by FISH signal
#337
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vreuter
opened
3 months ago
vreuter
commented
3 months ago
Use final step initially developed for DNA DSB project
Keep 1 row per spot, 1 column group per molecule of interest, 1 column per group for each measurement (e.g., mean and standard deviation)
Use, by default, just the central plane +/- 1 $z$-slice
Addition of fine drift correction for single (often initial) timepoint in which nuclei are imaged (now along with IF against proteins of interest)
Do this also for regional spots, in addition to obviously the locus-specific ones