Open yulicarp opened 6 months ago
Hi,
Thanks for your kind words, hope this helps:
Thanks for your reply!
Sorry to bother you again, could you tell me where i can download the full vector sequence?
I could only found the vector diagram in patent or article as shown below. Do I need to build this vector sequence myself using SnapGene?
Thanks in advance for your kind help!
https://patents.google.com/patent/WO2012079000A1/en https://www.nejm.org/doi/suppl/10.1056/NEJMoa1103849/suppl_file/nejmoa1103849_protocol.pdf
Kind regards, Yu Li
Hi Yu,
They have the sequence as part of the patent, see here: https://www.lens.org/lens/patent/175-246-371-138-874/sequences/view/1
Also if you're only interested in tisa-cel you may not need to do any sequence correction, most mismatches with our initial estimate came from axi-cel. Though worth checking the alignment in any case.
Nick
Hi,
thanks for this amazing work and also for making the code public.
I have some questions about generation of CAR reference: