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giannimonaco / ABIS

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number of input reads #1

Closed sdwien closed 5 years ago

sdwien commented 5 years ago

Dear Gianni, I would like to know how many read you used for the analysis in the paper, or how many reads you would consider the minimum number, a good number or more than enough for the analysis? Thanks and best, Sophia

giannimonaco commented 5 years ago

Dear Sophia,

The number of reads in the fastq files is between 15-20 millions. This is a good number for gene expression quantification. Check also https://support.illumina.com/bulletins/2017/04/considerations-for-rna-seq-read-length-and-coverage-.html

Best wishes,

Gianni