giannimonaco / flowAI

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Error in FUN(X[[i]], ...) : 'export_.....fcs' is not a valid file in flow_auto_qc()? #15

Open denvercal1234GitHub opened 1 year ago

denvercal1234GitHub commented 1 year ago

Hi there,

Thanks for the tool.

I ran flow_auto_qc() on untransformed FCS files (directly exported from FlowJo), but encountered the error below. Would you mind helping me troubleshoot?

Thank you.

export_F37_Live_flowAIresQC <- flow_auto_qc(F37_LiveSinglets_FCS)
Error in FUN(X[[i]], ...) :
'export_F37_Live.fcs' is not a valid file
> session_info()
─ Session info ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
 setting  value
 version  R version 4.2.2 (2022-10-31)
 os       macOS Ventura 13.1
 system   aarch64, darwin20
 ui       RStudio
 language (EN)
 collate  en_US.UTF-8
 ctype    en_US.UTF-8
 tz       Europe/London
 date     2023-01-26
 rstudio  2022.12.0+353 Elsbeth Geranium (desktop)
 pandoc   2.19.2 @ /Applications/RStudio.app/Contents/Resources/app/quarto/bin/tools/ (via rmarkdown)

─ Packages ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
 package              * version    date (UTC) lib source
 abind                  1.4-5      2016-07-21 [2] CRAN (R 4.2.0)
 askpass                1.1        2019-01-13 [2] CRAN (R 4.2.0)
 assertthat             0.2.1      2019-03-21 [1] CRAN (R 4.2.0)
 aws.s3                 0.3.21     2020-04-07 [2] CRAN (R 4.2.0)
 aws.signature          0.6.0      2020-06-01 [2] CRAN (R 4.2.0)
 backports              1.4.1      2021-12-13 [2] CRAN (R 4.2.0)
 base64enc              0.1-3      2015-07-28 [2] CRAN (R 4.2.0)
 beachmat               2.12.0     2022-04-26 [1] Bioconductor
 beeswarm               0.4.0      2021-06-01 [1] CRAN (R 4.2.0)
 Biobase              * 2.58.0     2022-11-07 [1] Bioconductor
 BiocGenerics         * 0.44.0     2022-11-07 [1] Bioconductor
 BiocManager            1.30.19    2022-10-25 [2] CRAN (R 4.2.0)
 BiocNeighbors          1.14.0     2022-04-26 [1] Bioconductor
 BiocParallel           1.30.4     2022-10-11 [1] Bioconductor
 BiocSingular           1.12.0     2022-04-26 [1] Bioconductor
 bitops                 1.0-7      2021-04-24 [2] CRAN (R 4.2.0)
 broom                  1.0.3      2023-01-25 [2] CRAN (R 4.2.0)
 bslib                  0.4.2      2022-12-16 [2] CRAN (R 4.2.0)
 cachem                 1.0.6      2021-08-19 [2] CRAN (R 4.2.0)
 callr                  3.7.3      2022-11-02 [2] CRAN (R 4.2.1)
 car                    3.1-1      2022-10-19 [2] CRAN (R 4.2.0)
 carData                3.0-5      2022-01-06 [2] CRAN (R 4.2.0)
 caret                * 6.0-93     2022-08-09 [2] CRAN (R 4.2.0)
 CATALYST             * 1.20.1     2022-05-22 [1] Bioconductor
 changepoint            2.2.4      2022-11-03 [1] CRAN (R 4.2.0)
 circlize               0.4.15     2022-05-10 [1] CRAN (R 4.2.0)
 class                * 7.3-21     2023-01-23 [2] CRAN (R 4.2.0)
 cli                    3.6.0      2023-01-09 [2] CRAN (R 4.2.0)
 clue                   0.3-63     2022-11-19 [2] CRAN (R 4.2.0)
 cluster                2.1.4      2022-08-22 [2] CRAN (R 4.2.2)
 codetools              0.2-18     2020-11-04 [2] CRAN (R 4.2.2)
 colorRamps           * 2.3.1      2022-05-02 [2] CRAN (R 4.2.0)
 colorspace             2.1-0      2023-01-23 [2] CRAN (R 4.2.0)
 ComplexHeatmap         2.14.0     2022-11-07 [1] Bioconductor
 ConsensusClusterPlus   1.60.0     2022-04-26 [2] Bioconductor
 corpcor                1.6.10     2021-09-16 [2] CRAN (R 4.2.0)
 cowplot                1.1.1      2020-12-30 [2] CRAN (R 4.2.0)
 crayon                 1.5.2      2022-09-29 [2] CRAN (R 4.2.0)
 curl                   5.0.0      2023-01-12 [2] CRAN (R 4.2.0)
 cytolib                2.8.0      2022-04-26 [2] Bioconductor
 CytoML                 2.8.1      2022-08-23 [2] Bioconductor
 CytoNorm             * 0.0.10     2023-01-18 [1] Github (saeyslab/CytoNorm@166f9ff)
 data.table           * 1.14.6     2022-11-16 [2] CRAN (R 4.2.0)
 DBI                    1.1.3      2022-06-18 [2] CRAN (R 4.2.0)
 DelayedArray           0.24.0     2022-11-07 [1] Bioconductor
 DelayedMatrixStats     1.18.2     2022-10-13 [1] Bioconductor
 deldir                 1.0-6      2021-10-23 [2] CRAN (R 4.2.0)
 DEoptimR               1.0-11     2022-04-03 [2] CRAN (R 4.2.0)
 deSolve                1.34       2022-10-22 [2] CRAN (R 4.2.0)
 devtools             * 2.4.5      2022-10-11 [2] CRAN (R 4.2.1)
 digest                 0.6.31     2022-12-11 [2] CRAN (R 4.2.0)
 doParallel             1.0.17     2022-02-07 [2] CRAN (R 4.2.0)
 dplyr                * 1.0.10     2022-09-01 [1] CRAN (R 4.2.0)
 drc                    3.0-1      2016-08-30 [1] CRAN (R 4.2.0)
 ellipsis               0.3.2      2021-04-29 [2] CRAN (R 4.2.0)
 evaluate               0.20       2023-01-17 [2] CRAN (R 4.2.0)
 fansi                  1.0.4      2023-01-22 [2] CRAN (R 4.2.0)
 farver                 2.1.1      2022-07-06 [2] CRAN (R 4.2.0)
 fastmap                1.1.0      2021-01-25 [2] CRAN (R 4.2.0)
 fda                    6.0.5      2022-07-04 [2] CRAN (R 4.2.0)
 fds                    1.8        2018-10-31 [2] CRAN (R 4.2.0)
 flowAI               * 1.26.0     2022-04-26 [1] Bioconductor
 flowCore             * 2.8.0      2022-04-26 [2] Bioconductor
 FlowSOM              * 2.4.0      2022-04-26 [2] Bioconductor
 flowStats            * 4.8.2      2022-07-12 [2] Bioconductor
 flowViz              * 1.60.2     2022-07-03 [2] Bioconductor
 flowVS               * 1.28.0     2022-04-26 [1] Bioconductor
 flowWorkspace          4.8.0      2022-04-26 [2] Bioconductor
 foreach                1.5.2      2022-02-02 [2] CRAN (R 4.2.0)
 fs                     1.6.0      2023-01-23 [2] CRAN (R 4.2.0)
 future                 1.30.0     2022-12-16 [2] CRAN (R 4.2.0)
 future.apply           1.10.0     2022-11-05 [2] CRAN (R 4.2.1)
 generics               0.1.3      2022-07-05 [2] CRAN (R 4.2.0)
 GenomeInfoDb         * 1.34.6     2023-01-04 [1] Bioconductor
 GenomeInfoDbData       1.2.9      2023-01-16 [1] Bioconductor
 GenomicRanges        * 1.50.2     2022-12-18 [1] Bioconductor
 GetoptLong             1.0.5      2020-12-15 [1] CRAN (R 4.2.0)
 ggbeeswarm             0.7.1      2022-12-16 [1] CRAN (R 4.2.0)
 ggcyto                 1.24.1     2022-07-03 [2] Bioconductor
 ggforce                0.4.1      2022-10-04 [2] CRAN (R 4.2.0)
 ggnewscale             0.4.8      2022-10-06 [2] CRAN (R 4.2.0)
 ggplot2              * 3.4.0      2022-11-04 [1] CRAN (R 4.2.1)
 ggpointdensity       * 0.1.0      2019-08-28 [2] CRAN (R 4.2.0)
 ggpubr               * 0.5.0      2022-11-16 [2] CRAN (R 4.2.2)
 ggrepel                0.9.2      2022-11-06 [2] CRAN (R 4.2.1)
 ggridges               0.5.4      2022-09-26 [2] CRAN (R 4.2.0)
 ggsignif               0.6.4      2022-10-13 [2] CRAN (R 4.2.0)
 ggthemes             * 4.2.4      2021-01-20 [2] CRAN (R 4.2.0)
 GlobalOptions          0.1.2      2020-06-10 [1] CRAN (R 4.2.0)
 globals                0.16.2     2022-11-21 [2] CRAN (R 4.2.2)
 glue                   1.6.2      2022-02-24 [2] CRAN (R 4.2.0)
 gower                  1.0.1      2022-12-22 [2] CRAN (R 4.2.0)
 graph                  1.74.0     2022-04-26 [2] Bioconductor
 gridExtra            * 2.3        2017-09-09 [2] CRAN (R 4.2.0)
 gtable                 0.3.1      2022-09-01 [2] CRAN (R 4.2.0)
 gtools                 3.9.4      2022-11-27 [2] CRAN (R 4.2.2)
 hardhat                1.2.0      2022-06-30 [2] CRAN (R 4.2.0)
 hdrcde                 3.4        2021-01-18 [2] CRAN (R 4.2.0)
 hexbin                 1.28.2     2021-01-08 [2] CRAN (R 4.2.0)
 highr                  0.10       2022-12-22 [2] CRAN (R 4.2.0)
 htmltools              0.5.4      2022-12-07 [2] CRAN (R 4.2.0)
 htmlwidgets            1.6.1      2023-01-07 [2] CRAN (R 4.2.0)
 httpuv                 1.6.8      2023-01-12 [2] CRAN (R 4.2.0)
 httr                   1.4.4      2022-08-17 [2] CRAN (R 4.2.0)
 IDPmisc                1.1.20     2020-01-21 [2] CRAN (R 4.2.0)
 igraph               * 1.3.5      2022-09-22 [2] CRAN (R 4.2.0)
 interp                 1.1-3      2022-07-13 [2] CRAN (R 4.2.0)
 ipred                  0.9-13     2022-06-02 [2] CRAN (R 4.2.0)
 IRanges              * 2.32.0     2022-11-07 [1] Bioconductor
 irlba                  2.3.5.1    2022-10-03 [2] CRAN (R 4.2.0)
 iterators              1.0.14     2022-02-05 [2] CRAN (R 4.2.0)
 jpeg                   0.1-10     2022-11-29 [2] CRAN (R 4.2.0)
 jquerylib              0.1.4      2021-04-26 [2] CRAN (R 4.2.0)
 jsonlite               1.8.4      2022-12-06 [2] CRAN (R 4.2.0)
 KernSmooth             2.23-20    2021-05-03 [2] CRAN (R 4.2.2)
 knitr                * 1.42       2023-01-25 [1] CRAN (R 4.2.2)
 ks                     1.14.0     2022-11-24 [2] CRAN (R 4.2.2)
 labeling               0.4.2      2020-10-20 [2] CRAN (R 4.2.0)
 later                  1.3.0      2021-08-18 [2] CRAN (R 4.2.0)
 lattice              * 0.20-45    2021-09-22 [2] CRAN (R 4.2.2)
 latticeExtra           0.6-30     2022-07-04 [2] CRAN (R 4.2.0)
 lava                   1.7.1      2023-01-06 [2] CRAN (R 4.2.0)
 lifecycle              1.0.3.9000 2022-10-17 [2] Github (r-lib/lifecycle@80a1e52)
 listenv                0.9.0      2022-12-16 [2] CRAN (R 4.2.0)
 lubridate              1.9.1      2023-01-24 [2] CRAN (R 4.2.0)
 magrittr               2.0.3      2022-03-30 [2] CRAN (R 4.2.0)
 MASS                   7.3-58.2   2023-01-23 [1] CRAN (R 4.2.0)
 Matrix               * 1.5-3      2022-11-11 [1] CRAN (R 4.2.1)
 MatrixGenerics       * 1.10.0     2022-11-07 [1] Bioconductor
 matrixStats          * 0.63.0     2022-11-18 [2] CRAN (R 4.2.0)
 mclust                 6.0.0      2022-10-31 [2] CRAN (R 4.2.0)
 memoise                2.0.1      2021-11-26 [2] CRAN (R 4.2.0)
 mime                   0.12       2021-09-28 [2] CRAN (R 4.2.0)
 miniUI                 0.1.1.1    2018-05-18 [2] CRAN (R 4.2.0)
 mnormt                 2.1.1      2022-09-26 [2] CRAN (R 4.2.0)
 ModelMetrics           1.2.2.2    2020-03-17 [2] CRAN (R 4.2.0)
 multcomp               1.4-20     2022-08-07 [1] CRAN (R 4.2.0)
 munsell                0.5.0      2018-06-12 [2] CRAN (R 4.2.0)
 mvtnorm                1.1-3      2021-10-08 [2] CRAN (R 4.2.0)
 ncdfFlow               2.42.1     2022-07-03 [2] Bioconductor
 nlme                   3.1-161    2022-12-15 [2] CRAN (R 4.2.0)
 nnet                   7.3-18     2022-09-28 [2] CRAN (R 4.2.2)
 nnls                   1.4        2012-03-19 [1] CRAN (R 4.2.0)
 openssl                2.0.5      2022-12-06 [2] CRAN (R 4.2.0)
 parallelly             1.34.0     2023-01-13 [2] CRAN (R 4.2.0)
 pcaPP                  2.0-3      2022-10-24 [2] CRAN (R 4.2.1)
 PeacoQC              * 1.6.0      2022-04-26 [1] Bioconductor
 pheatmap             * 1.0.12     2019-01-04 [2] CRAN (R 4.2.0)
 pillar                 1.8.1      2022-08-19 [2] CRAN (R 4.2.0)
 pkgbuild               1.4.0      2022-11-27 [2] CRAN (R 4.2.2)
 pkgconfig              2.0.3      2019-09-22 [2] CRAN (R 4.2.0)
 pkgload                1.3.2      2022-11-16 [2] CRAN (R 4.2.0)
 plotrix                3.8-2      2021-09-08 [1] CRAN (R 4.2.0)
 plyr                 * 1.8.8      2022-11-11 [2] CRAN (R 4.2.1)
 png                    0.1-8      2022-11-29 [2] CRAN (R 4.2.0)
 polyclip               1.10-4     2022-10-20 [2] CRAN (R 4.2.0)
 pracma                 2.4.2      2022-09-22 [2] CRAN (R 4.2.0)
 prettyunits            1.1.1      2020-01-24 [2] CRAN (R 4.2.0)
 pROC                   1.18.0     2021-09-03 [2] CRAN (R 4.2.0)
 processx               3.8.0      2022-10-26 [2] CRAN (R 4.2.0)
 prodlim                2019.11.13 2019-11-17 [2] CRAN (R 4.2.0)
 profvis                0.3.7      2020-11-02 [2] CRAN (R 4.2.0)
 promises               1.2.0.1    2021-02-11 [2] CRAN (R 4.2.0)
 ps                     1.7.2      2022-10-26 [2] CRAN (R 4.2.0)
 purrr                  1.0.1      2023-01-10 [2] CRAN (R 4.2.0)
 R6                     2.5.1      2021-08-19 [2] CRAN (R 4.2.0)
 rainbow                3.7        2022-10-09 [2] CRAN (R 4.2.0)
 RBGL                   1.72.0     2022-04-26 [2] Bioconductor
 RColorBrewer         * 1.1-3      2022-04-03 [2] CRAN (R 4.2.0)
 Rcpp                   1.0.10     2023-01-22 [2] CRAN (R 4.2.0)
 RcppParallel           5.1.6      2023-01-09 [2] CRAN (R 4.2.0)
 RCurl                  1.98-1.9   2022-10-03 [2] CRAN (R 4.2.0)
 recipes                1.0.4      2023-01-11 [2] CRAN (R 4.2.0)
 remotes                2.4.2      2021-11-30 [2] CRAN (R 4.2.0)
 reshape2               1.4.4      2020-04-09 [2] CRAN (R 4.2.0)
 reticulate           * 1.27       2023-01-07 [2] CRAN (R 4.2.0)
 Rgraphviz              2.40.0     2022-04-26 [2] Bioconductor
 rJava                  1.0-6      2021-12-10 [1] CRAN (R 4.2.0)
 rjson                  0.2.21     2022-01-09 [1] CRAN (R 4.2.0)
 rlang                  1.0.6      2022-09-24 [2] CRAN (R 4.2.0)
 rmarkdown              2.20       2023-01-19 [2] CRAN (R 4.2.0)
 robustbase             0.95-0     2022-04-02 [2] CRAN (R 4.2.0)
 rpart                  4.1.19     2022-10-21 [2] CRAN (R 4.2.2)
 RProtoBufLib           2.8.0      2022-04-26 [2] Bioconductor
 rrcov                  1.7-2      2022-10-24 [2] CRAN (R 4.2.1)
 RSpectra               0.16-1     2022-04-24 [2] CRAN (R 4.2.0)
 rstatix                0.7.1      2022-11-09 [2] CRAN (R 4.2.1)
 rstudioapi           * 0.14       2022-08-22 [2] CRAN (R 4.2.0)
 rsvd                   1.0.5      2021-04-16 [2] CRAN (R 4.2.0)
 Rtsne                * 0.16       2022-04-17 [2] CRAN (R 4.2.0)
 S4Vectors            * 0.36.1     2022-12-07 [1] Bioconductor
 sandwich               3.0-2      2022-06-15 [1] CRAN (R 4.2.0)
 sass                   0.4.5      2023-01-24 [2] CRAN (R 4.2.0)
 ScaledMatrix           1.4.1      2022-09-11 [1] Bioconductor
 scales               * 1.2.1      2022-08-20 [2] CRAN (R 4.2.0)
 scater                 1.24.0     2022-04-26 [1] Bioconductor
 scattermore            0.8        2022-02-14 [2] CRAN (R 4.2.0)
 scuttle                1.6.3      2022-08-23 [1] Bioconductor
 sessioninfo            1.2.2      2021-12-06 [2] CRAN (R 4.2.0)
 shape                  1.4.6      2021-05-19 [1] CRAN (R 4.2.0)
 shiny                  1.7.4      2022-12-15 [2] CRAN (R 4.2.0)
 SingleCellExperiment * 1.20.0     2022-11-07 [1] Bioconductor
 sparseMatrixStats      1.8.0      2022-04-26 [1] Bioconductor
 Spectre              * 1.0.0      2022-10-19 [2] Github (immunedynamics/spectre@3d0c002)
 stringi                1.7.12     2023-01-11 [2] CRAN (R 4.2.0)
 stringr                1.5.0      2022-12-02 [2] CRAN (R 4.2.0)
 SummarizedExperiment * 1.28.0     2022-11-07 [1] Bioconductor
 survival               3.5-0      2023-01-09 [2] CRAN (R 4.2.0)
 TH.data                1.1-1      2022-04-26 [1] CRAN (R 4.2.0)
 tibble                 3.1.8      2022-07-22 [2] CRAN (R 4.2.0)
 tidyr                * 1.3.0      2023-01-24 [2] CRAN (R 4.2.0)
 tidyselect             1.2.0      2022-10-10 [2] CRAN (R 4.2.1)
 timechange             0.2.0      2023-01-11 [2] CRAN (R 4.2.0)
 timeDate               4022.108   2023-01-07 [2] CRAN (R 4.2.0)
 tweenr                 2.0.2      2022-09-06 [2] CRAN (R 4.2.0)
 umap                 * 0.2.9.0    2022-08-25 [2] CRAN (R 4.2.0)
 urlchecker             1.0.1      2021-11-30 [2] CRAN (R 4.2.0)
 usethis              * 2.1.6      2022-05-25 [2] CRAN (R 4.2.0)
 utf8                   1.2.2      2021-07-24 [2] CRAN (R 4.2.0)
 uwot                 * 0.1.14     2022-08-22 [1] CRAN (R 4.2.0)
 vctrs                  0.5.2      2023-01-23 [1] CRAN (R 4.2.0)
 vipor                  0.4.5      2017-03-22 [1] CRAN (R 4.2.0)
 viridis                0.6.2      2021-10-13 [2] CRAN (R 4.2.0)
 viridisLite            0.4.1      2022-08-22 [2] CRAN (R 4.2.0)
 withr                  2.5.0      2022-03-03 [2] CRAN (R 4.2.0)
 xfun                   0.36       2022-12-21 [2] CRAN (R 4.2.0)
 xlsx                 * 0.6.5      2020-11-10 [1] CRAN (R 4.2.0)
 xlsxjars               0.6.1      2014-08-22 [1] CRAN (R 4.2.0)
 XML                    3.99-0.13  2022-12-04 [2] CRAN (R 4.2.0)
 xml2                   1.3.3      2021-11-30 [2] CRAN (R 4.2.0)
 xtable                 1.8-4      2019-04-21 [2] CRAN (R 4.2.0)
 XVector                0.38.0     2022-11-07 [1] Bioconductor
 yaml                   2.3.7      2023-01-23 [2] CRAN (R 4.2.0)
 zlibbioc               1.44.0     2022-11-07 [1] Bioconductor
 zoo                    1.8-11     2022-09-17 [2] CRAN (R 4.2.0)

 [1] /Users/clusteredatom/Library/R/arm64/4.2/library
 [2] /Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/library
giannimonaco commented 1 year ago

Hi, I am not sure about this issue. Did you also try to run the QC on the original FCS file (not the one exported by flowJo)? Did you also try other files?

On Thu, Jan 26, 2023 at 8:51 PM Quang Nguyen @.***> wrote:

Hi there,

Thanks for the tool.

I ran flow_auto_qc() on untransformed FCS files (directly exported from FlowJo), but encountered the error below. Would you mind helping me troubleshoot?

Thank you.

export_F37_Live_flowAIresQC <- flow_auto_qc(F37_LiveSinglets_FCS)

Error in FUN(X[[i]], ...) :

'export_F37_Live.fcs' is not a valid file

session_info()

─ Session info ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────

setting value

version R version 4.2.2 (2022-10-31)

os macOS Ventura 13.1

system aarch64, darwin20

ui RStudio

language (EN)

collate en_US.UTF-8

ctype en_US.UTF-8

tz Europe/London

date 2023-01-26

rstudio 2022.12.0+353 Elsbeth Geranium (desktop)

pandoc 2.19.2 @ /Applications/RStudio.app/Contents/Resources/app/quarto/bin/tools/ (via rmarkdown)

─ Packages ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────

package * version date (UTC) lib source

abind 1.4-5 2016-07-21 [2] CRAN (R 4.2.0)

askpass 1.1 2019-01-13 [2] CRAN (R 4.2.0)

assertthat 0.2.1 2019-03-21 [1] CRAN (R 4.2.0)

aws.s3 0.3.21 2020-04-07 [2] CRAN (R 4.2.0)

aws.signature 0.6.0 2020-06-01 [2] CRAN (R 4.2.0)

backports 1.4.1 2021-12-13 [2] CRAN (R 4.2.0)

base64enc 0.1-3 2015-07-28 [2] CRAN (R 4.2.0)

beachmat 2.12.0 2022-04-26 [1] Bioconductor

beeswarm 0.4.0 2021-06-01 [1] CRAN (R 4.2.0)

Biobase * 2.58.0 2022-11-07 [1] Bioconductor

BiocGenerics * 0.44.0 2022-11-07 [1] Bioconductor

BiocManager 1.30.19 2022-10-25 [2] CRAN (R 4.2.0)

BiocNeighbors 1.14.0 2022-04-26 [1] Bioconductor

BiocParallel 1.30.4 2022-10-11 [1] Bioconductor

BiocSingular 1.12.0 2022-04-26 [1] Bioconductor

bitops 1.0-7 2021-04-24 [2] CRAN (R 4.2.0)

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[1] /Users/clusteredatom/Library/R/arm64/4.2/library

[2] /Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/library

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