giannimonaco
commented
2 years ago Hi! Thank you for reporting this! You are right, the Min and Max range values were converted to characters for output=2. I just fixed it. Just use the github version if you need the fixed version soon.
On Tue, 30 Nov 2021 at 12:57, Tim Mocking @.***> wrote:
A small suggestion: flowAI converts the Min and Max range as defined in the .FCS parameters to characters. This is normally not an issue, as saving the .FCS re-converts these to doubles. However, if you run an additional anomaly detection algorithm after running flowAI, the output is erroneous because of this.
library(flowAI) library(flowCore)
ff <- read.FCS(FILE) ff <- flow_auto_qc(ff, output=2)
meta <- flowWorkspace::pData(flowCore::parameters(ff)) typeof(meta['FSC-A', 'minRange'])
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A small suggestion: flowAI converts the Min and Max range as defined in the .FCS parameters to characters. This is normally not an issue, as saving the .FCS re-converts these to doubles. However, if you run an additional anomaly detection algorithm after running flowAI, the output is erroneous because of this.