DW-PBJ2-Paige

[paige-ramkissoon]

Discussion worksheet

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Main Research Question?

The researchers were interested in looking at how the lung immune system is effected by COVID infection and understanding the immune cascade with infection.

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Methods & Data

The researchers in this study utilized several methods to investigate their question. Humanized mouse models were used in an attempt to understand how COVID effect humans rather than mice. With the animal models they collected cells to perform in vitro studies. Flow cytometry, RNA analysis, and histological techniques were all used as well.

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Authors' interpretation

I agree with the authors' interpretation because the data they present supports what they are saying.

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Enhancement or next experiment?

I think it would be interesting to see how COVID effects other humanized mouse models or models with other disease or dysfunctions, like COPD, asthma, lung cancer, etc.

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confusion? Terms you don't understand?

I don't think I have any.

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Have your own opinions or critiques

I would have like to see them explain their criteria for their histological scores.

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I hereby confirm that I have:

• [ ] I am still working on the worksheet
• [x] I have finished the worksheet
• [x] I have gone through the papers
• [x] Check your peer if they have similar ideas. If it is, plz add their issue number to your comments.

[gmhhope]

I would have like to see them explain their criteria for their histological scores.

Could you find any related papers and share them here (for example, link more if you have)? It will be interesting for everyone too. As you may aware, the scoring system to diagnose or stratify patients with certain diseases is highly complex. The scope of this paper definitely will not touch on this aspect but I think it is highly relevant for you guys looking for MD/PHD program

I am dealing with some ME/CFS patients which involved multiple questionnaires (e.g., Pittsburg Sleep Quality Index, PEM Assessment) and Quest Lab results. Yet, it is still in the air how we can diagnose these patients as they are highly heterogenous.

or models with other diseases or dysfunctions, like COPD, asthma, lung cancer, etc.

This is exactly what we are trying to explore in the next half sessions of PBJ. I saw you are going to prepare for Multisystem inflammatory syndrome in children (MIS-C). I think that will be one of an important aspect to look into what scoring system or consensus for diagnosing this rare but devastating disease.

Humanized mouse models

• Humanized mouse model will not be thoroughly discussed during our PBJ session as it is used mostly on the back-2-back paper focusing on macrophages. But if you think it will be very relevant, you can share your thoughts during the meeting which I will present one slide touching on this aspect

[paige-ramkissoon]

https://infectagentscancer.biomedcentral.com/articles/10.1186/s13027-021-00369-0 This is a good review paper that looks at several papers on COVID. They used 24 papers looking at the lungs and the histopathological scores. Some papers include great deal of detail explaining what they find and include in their scores, while others are vague.

[gmhhope]

If you wish, I could have 2-3 min for you to explain to us briefly the scoring system. Let me know and send me your slides if you wish (just need 1-2 slides)! Appreciate it!

[paige-ramkissoon]

I've been trying to find some papers that explain histopathological scores but most papers use a scoring system from 0-3. This paper uses a scoring system from 0-5, they said they had a board certified pathologist blindly score the images.

[gmhhope]

I've been trying to find some papers that explain histopathological scores but most papers use a scoring system from 0-3. This paper uses a scoring system from 0-5, they said they had a board certified pathologist blindly score the images.

So is COVID-19 severity classification completely based on histopathological scores? Thanks!