DW-PBJ4-Zijian

[zijian-li1228]

Discussion worksheet

The paper you are working on?

GeurtsvanKessel et al.

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Main Research Question?

How does antibody persistence and neutralizing activity against SARS-CoV-2 variants differ for people received AstraZeneca, Janssen, Moderna, or Pfizer vaccination.

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Methods & Data

• antibody levels drop for most vaccines. T cell response is higher for mRNA based vaccines (ELISA based assays)
• at 6 mo post vaccination, neutralizing antibodies against WT drops for all except Ad26.COV2.S vaccination. Significantly lower neutralization of omicron variant was observe across all vaccinations (PRNT)
• CD4+ and CD8+ T cell response to WT, delta, beta and omicron were not significantly different in BNT162B2 vaccinated individuals (flow cytometry)
• neutralizing antibody and T cells response against omicron variant increase after heterologous booster

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Authors' interpretation

I agree with the authors' interpretation.

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Enhancement or next experiment?

increase sample size and stratify data by age/biological sex/underlying conditions to minimize confounding variables.

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Confusion? Terms you don't understand?

I'm confused by their blood sample collection point (ie different number of days post vaccination, especially for figure 4) and will that affect the validity of the study?

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Have your own opinions or critiques

figure 4 would be more convincing if they include comparison for both homologous and heterologous boosters?

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I hereby confirm that I have:

• [ ] I am still working on the worksheet
• [x] I have finished the worksheet
• [x] I have gone through the papers
• [x] Check your peer if they have similar ideas. If it is, plz add their issue number to your comments.

[gmhhope]

increase sample size and stratify data by age/biological sex/underlying conditions to minimize confounding variables.

That is a very important pitfall for this study.

figure 4 would be more convincing if they include comparison for both homologous and heterologous boosters?

Good point! If this is going for a higher journal, this will be definitely asked. I think many results were supported by many other reports published in the earlier wave.

I'm confused by their blood sample collection point (ie different number of days post vaccination, especially for figure 4) and will that affect the validity of the study?

I think that is based on how many doses of vaccine you received. They rationalized probably by (1) 1-dose: 8 weeks; (2)2-dose: 4 weeks; 3-dose: 2 weeks. The rationale is more or less OK but it is also subjected for debation.